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Can I get a free sample for a trial？
Yes, Sino Biological has free samples available from time to time in our promotion activities. Please visit www.sinobiological.com frequently or contact us directly.
Can I get discount for Sino Biological products？
In general, Sino Biological provides special discounts for bulk orders and some new products. In addition we have sales promotion from time to time for various antibody reagents. If you would like to get the information, please visit our website( www.sinobiological.com) frequently, contact our customer service (400-800-9989 - Order) or our local authorized distributers.
Which kinds of antibodies does Sino Biological have? How can I choose the right one?
Sino Biological provides a wide variety of antibody products to meet different customer's requests including affinity and non-affinity purified rabbit polyclonal antibodies, rabbit and mouse monoclonal antibodies, and fluorescence or enzyme conjugated antibodies. As miscellaneous proteins existed in non-purified antibodies may affect the antibody performance in some assays we do not offer any antibody products without purification such as serum, culture supernatant or ascites.
Sometimes Sino Biological offers more than one antibody product against the same target. You need choose the antibody to meet your experimental requirements based on antibody application, specificity， cross-reactivity, species, and immunogens. Please contact our customer service (400-800-9989 - Order) for assistance in selecting the best fit antibody for you.
Where can I get the reactivity of antibody with other species? How do I determine whether an antibody can be used to detect the antigen from other species?
Due to the complex antigen structure and modification it is hard to estimate the antibody binding to the antigens from other species. Even if the sequence of antigen among different species is very similar, the recognition of the antibody is not guaranteed.
Sino Biological's products provide some data regarding antibody cross-reactivity to determine whether such antibodies can recognize protein isoforms ，other members in the same superfamily and target antigen from other species. Please note, however, that the data for cross-reactions are mostly obtained by ELISA, they may not apply to other applications such as Western Blotting and IHC.
If you could feedback relevant results of antibody in untested species to Sino Biological (either negative or positive), we will provide you a special promotion or award. Of course, in order to enhance the possibility for unknown species, comparison of the amino acid sequence between immunogen and the tested target is recommended.
Does Sino Biological provide antibodies with multiple applications?
For the same antigen, more than one application is required for the corresponding antibody by some customers. Based on Sino Biological antibody screening strategy, for each application, only the best one is for sale. Since different applications have their specific requirements to the antibodies it is hard to find the antibodies meeting all applications. However, we do have some antibodies with more than one application and will try our best to provide more and more multi-functional antibodies in the future.
Will Sino Biological Inc.'s antibody work in an application that is not listed in the applications table?
Customers can find all the tested applications for each particular antibody by visiting Sino Biological Inc. Antibody website. We do not list any untested applications. We would greatly appreciate it if you would test our antibody in an unproven application and share the results with us. We will issue you credits or awards in return for your data.
What is the particular monoclonal antibody epitope? Is the antigen sequence provided?
Many of our antibodies are produced using recombinant proteins, so it is hard to identify the specific epitope of the monoclonal antibody. The catalog number of the recombinant protein used as the immunogen is listed on the Certificate of Analysis. For the monoclonal antibodies obtained using peptides as antigens, an epitope range recognized by corresponding antibody will be provided.
Is rabbit monoclonal antibody better than mouse monoclonal antibody?
The affinity of rabbit monoclonal antibody is 10-100 times higher than that of mouse antibody. Rabbit monoclonal antibody is highly recommended if you have following requirements:
- Need excellent performance in immunohistochemistry or Western blotting applications;
- Requirement in detection of targets with low expression level;
- Develop ELISA assays with other mouse monoclonal antibodies.
How can I select the right secondary antibody for my experiment?
In general, secondary antibody refers to it can bind to primary antibody and is labeled with a marker or dye (e.g. fluorescent dye, radioactive molecule, chemiluminescent, or chromogenic groups). With secondary antibody, the signal can be amplified for detection. We suggest you consider the following factors:
1. Depending on the host species. For example, if the primary antibody is from rabbit, the secondary antibody should be anti-rabbit.
2. Depending on the characteristics of primary antibody. If the primary antibody is a Fab fragment, you can only select the anti-Fab fragment secondary antibody or anti-full length secondary antibody products, but not anti-Fc fragment secondary antibody.
3. Depending on your applications, such as WB, IHC, ELISA, etc. Please refer our data sheet to confirm the secondary antibody tested for the related applications. Currently, Sino Biological provides HRP, biotin and fluorescent dye-labeled secondary antibody. The secondary antibody with more labels will be on our list soon.
What is the antibody clone ID? What is the difference between clone ID and lot number?
Antibody clone ID is the internal unique identity number assigned to each monoclonal antibody clone while lot number is corresponding to each batch of antibody mass production. The antibodies with same clone ID but different lot numbers have no difference in general.
What materials are provided in your ELISA Kit and ELISA pair sets?
Currently our ELISA pair sets contain the capture antibody, the detection antibody, and the standard (recombinant protein). Other buffer solutions and substrate stock solutions required for the development of sandwich ELISAs need to be prepared by customers. In contrast, our complete ELISA kits provide all above reagents and are ready to use for your ELISA experiments.
Could Sino Biological develop an antibody for me if I do not find the suitable catalogue products?
Yes. Sino Biological provide one-stop antibody services for all steps of antibody development and production, including peptide and recombinant protein antigen preparation, animal immunization, mouse hybridoma fusion, rabbit polyclonal and monoclonal antibody development, antibody production from mg to 100 grams, and comprehensive antibody validations for multiple applications. Please call us or send an email to firstname.lastname@example.org for more information.
Can I ask for a replacement or refund for your antibody products?
Sino Biological is committed to provide high quality products. If you find quality problems, the products can be replaced for free or you will get a full refund. However, if the antibody used in untested species or application, we can not provide a replacement or refund. Please read the product description or contact our customer service staff carefully before purchase.