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Custom Recombinant Protein Expression Services

Why choose Sino Biological for your protein production service?

• Sino has over 10 years' experience resulting in the successful development and production of 6000+ recombinant proteins.
• One-stop service from gene to protein
Multiple protein expression systems available including bacteria, yeast, baculovirus-insect and mammalian expression systems
• Multiple purification systems (30+) from which to choose
• Comprehensive research and development capabilities with over 1000 new proteins  generated per year
• Almost 100 bioreactors with various volume capacities ranging from 2 to 1000 L to meet high-throughput and large-scale production requirements

Overview of Recombinant Protein Expression: Key Influencing Factors, Case Studies and FAQs
Download Sino Biological's newest Brochure (2019):
Strategies to Obtain High-Quality Recombinant Proteins: Protocols and Case Studies

All protein production services

  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Expression evaluation
    • Protein expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Expression and purification
    • Large-scale expression and purification (optional)
  • • Gene synthesis and codon optimization (optional)
    • Vector construction
    • Virus packaging and amplification
    • Expression and purification
    • Large-scale expression and purification (optional)

Case Studies of Recombinant Protein Production Service Case Studies

Case study of E.coli protein A expression     More details>>>

  • SEC-HPLC >98.5%
  • Cell bioactivity: EC50=17pg/mL

Case study of Baculovirus-Insect protein A expression     More details>>>

  • SEC-HPLC >93%
  • Cell bioactivity: EC50=7ng/mL

Case study of HEK293 transient protein A expression     More details>>>

  • SEC-HPLC >98.2%
  • SDS-PAGE >98.7%

Custom Recombinant Protein Production Service Advantages

E.coli Protein Expression Service • Over 10 years' experience of protein expression
• Multiple protein purification and refolding technologies
• High-throughput and large-scale protein expression and production
• One-stop service from gene to purified protein
• Multiple expression vectors and E.coli cells to meet various expression needs
Mammalian Transient Protein Expression Service • High-efficiency expression vectors
• Optimized mammalian expression systems
• High-throughput and large-scale production
• High yield expression with post-translational modification and correct folding
• One-stop service from gene sequence to purified protein
Baculovirus-Insect Cell Protein Expression Service • High-efficiency expression of intracellular and extracellular proteins
• Multiple insect cells: Sf9,Sf21,Hi-5
• High-throughput and large-scale protein production
• Optimized expression vectors and higher-titre virus packaging

Recombinant Protein Production Systems Reference

Expression Systems E.coli Yeast Baculovirus-insect Mammalian
Prokaryotic Eukaryotic Eukaryotic Eukaryotic
Advantages • Low cost
• Rapid expression
• Easy scale-up
• Most widely used system for recombinant protein production
• Low cost 
• Rapid expression 
• High yield
• Diverse post-translational modifications
• High capacity genes 
• Soluble proteins
• Suitable for toxic proteins
• Post-translational modifications similar to mammalian systems
• Soluble protein
• Lower endotoxin 
• Better bioactivity
• Comprehensive post-translational modifications 
• Transient & stable expression
Challenges • Inclusion bodies
• Lack of eukaryotic post-translational modifications
• Difficult to express higher MW proteins
• Improper glycosylation 
• High mannose modification
• More demanding culture conditions
• Lack of partial glycosylation
• More demanding culture conditions
Most Common Applications • Bacterial proteins
• Antigen proteins
Cytokines
• Enzymes
Cytokines
• Lower MW proteins
• Enzymes
• Cytoplasmic proteins 
• Toxic proteins
• Transmembrane proteins
• Secretory proteins
Kinases
• Secretory proteins
• Extracellular domain of transmembrane proteins
Recombinant antibodies
• antibody fragments

Contact Us

Tel
+86-010-50911666
Fax
+86-010-50953280
Email
cro-service@sinobiological.com