Anti-S100A6 Antibody

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Anti-S100A6 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-S100A6 Antibody
Validated applications
WB,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human S100A6
Immunogen
Recombinant Human S100A6 protein (Catalog#10939-HNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Human S100A6 (rh S100A6; Catalog#10939-HNAE; NP_055439.1; Met 1-Gly 90). S100A6 specific IgG was purified by Human S100A6 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-S100A6 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
IHC-P 1:5000-1:20000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-S100A6 Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human S100A6 in human renal carcinoma (from 3 donors) with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human S100A6 in human lung cancer with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human S100A6 in human hepatoma (from 2 donors) with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human CK14 in rat skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

S100A6 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

2 µL anti-S100A6 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-S100A6 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 12 kDa

Observed band size: 12 kDa

Anti-S100A6 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:10 kDa

Observed band size:10 kDa

Anti-S100A6 Antibody: Alternative Names

Anti-2A9 Antibody; Anti-5B10 Antibody; Anti-CABP Antibody; Anti-CACY Antibody; Anti-PRA Antibody

S100A6 Background Information

S1 proteinis a family of low molecular weight protein found in vertebrates characterized by twoEF-hand calcium-binding motifs. There are at least 21 different S1 proteins, and the name is derived from the fact that the protein is1%soluble in ammonium sulfateat neutralpH. Most S1 proteins are disulfide-linked homodimer, and is normally present in cells derived from theneural crest, chondrocytes, macrophages, dendritic cells, etc. S1 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S1A6 (S1 calcium binding protein A6) is a member of the S1 family of proteins, and functions in prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S1A6 have been implicated in melanoma.
Full Name
S100 calcium binding protein A6
References
  • Schäfer, B.W. et al., 1996, Trends Biochem. Sci. 21 (4): 134-140.
  • Donato,R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-551.
  • Nowotny, M. et al., 2003, J. Biol. Chem. 278 (29): 26923-26928.
  • Nonaka D, et al., 2008, J. Cutan. Pathol. 35 (11): 1014-1019.
  • Marenholz, I. et al., 2004, Biochem. Biophys. Res. Commun. 322 (4): 1111-22. 
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