Tissue Factor Protein Overview: Sequence, Structure, Function and Protein Interaction

Tissue Factor Protein Overview

The extrinsic pathway of blood coagulation is initiated by contact of plasma factor VII (F7; 613878) with tissue factor (F3), a cellular membrane glycoprotein that normally is segregated from the bloodstream but can be exposed after tissue injury or newly synthesized in endothelial cells or leukocytes after stimulation by endotoxin and cytokines. Once formed, the tissue factor/factor VII complex converts to a complex of tissue factor and enzymatically active factor VII (F7a); this conversion leads to activation of factors X (F10; 613872) and IX (300746) and prothrombin (176930), and ultimately to formation of fibrin (134570/134580). These processes are regulated by plasma protease inhibitors and by the thrombomodulin (188040)-protein C (612283) pathway. A further regulatory mechanism in the extrinsic pathway is called tissue factor pathway inhibitor (TFPI; 152310). For a review, see Davie et al. (1991).

Tissue Factor protein family

Belongs to the tissue factor family.

Tissue Factor protein name

Recommended name
Tissue factor
Short name
TF
Aliases
CD142, TF, tissue factor
Alternative name
Coagulation factor III Thromboplastin CD_antigen: CD142

Tissue Factor Protein Sequence

Species Human Tissue Factor protein
Length 295
Mass (Da) 33068
Sequence Human Tissue Factor protein sequence
Species Mouse Tissue Factor protein
Length 294
Mass (Da) 32935
Sequence Mouse Tissue Factor protein sequence
Species Rat Tissue Factor protein
Length 295
Mass (Da) 33444
Sequence Rat Tissue Factor protein sequence

Tissue Factor Protein Molecular Weight & PI

Tissue factor precursor (TF) (Coagulation factor III) (Thromboplastin) (CD142 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 33-295 Tissue factor.

Molecular weight (Da)

29593.43

Theoretical pI

6.01

Tissue Factor Protein Structure

EXTRACELLULAR DOMAIN OF HUMAN TISSUE FACTOR
Deposited
1997-04-10   Released:  1998-02-25
Deposition Author(s)
Huang, M., Syed, R., Stura, E.A., Stone, M.J., Stefanko, R.S., Ruf, W., Edgington, T.S., Wilson, I.A.
Organism(s)
Homo sapiens
Expression System
Escherichia coli BL21(DE3)
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.4000 Å
R-Value Free
0.297
R-Value Work
0.217
1TFH From PDB

Human Tissue Factor protein Secondary structure

Tissue Factor Protein Interaction

Recombinant Tissue Factor Protein Feature

Tissue Factor Protein, Human, Recombinant (hFc Tag)

High Purity
> 92 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method

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