NBL1 Protein Overview: Sequence, Structure, Function and Protein Interaction

NBL1 Protein Overview

The NBL1 gene, also known as DAN (for 'differential screening-selected gene aberrant in neuroblastoma'), was originally cloned from a normal rat fibroblast cDNA library by a differential screening method (Ozaki and Sakiyama, 1993). Enomoto et al. (1994) isolated the human DAN counterpart from a normal lung cDNA library by using rat DAN cDNA as a probe. They determined that the DAN gene encodes a transcription factor homologous to a mouse tumor suppressor gene.

NBL1 protein family

Belongs to the DAN family.

NBL1 protein name

Recommended name
Neuroblastoma suppressor of tumorigenicity 1
Aliases
D1S1733E, DAN, DAND1, differential screening-selected gene aberrant in neuroblastoma, NB, neuroblastoma candidate region, suppression of tumorigenicity 1, neuroblastoma suppressor of tumorigenicity 1, NO3
Alternative name
DAN domain family member 1 Protein N03 Zinc finger protein DAN

NBL1 Gene family protein

NBL1 Protein Sequence

Species Human NBL1 protein
Length 181
Mass (Da) 19408
Sequence Human NBL1 protein sequence
Species Mouse NBL1 protein
Length 178
Mass (Da) 19107
Sequence Mouse NBL1 protein sequence
Species Rat NBL1 protein
Length 178
Mass (Da) 19191
Sequence Rat NBL1 protein sequence

NBL1 Protein Molecular Weight & PI

Neuroblastoma suppressor of tumorigenicity 1 precursor (DAN domain family member 1) (Protein N03) (Zinc finger protein DAN) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 17-181 Neuroblastoma suppressor of

Molecular weight (Da)

17698.67

Theoretical pI

5.03

NBL1 Protein Structure

X-ray crystal structure of the dimeric BMP antagonist NBL1
Deposited
2014-11-24   Released:  2015-01-14
Deposition Author(s)
Thompson, T.B., Nolan, K., Kattamuri, C.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.5000 Å
R-Value Free
0.273
R-Value Work
0.216
4X1J From PDB

Human NBL1 protein Secondary structure

NBL1 Protein Interaction

Recombinant NBL1 Protein Feature

NBL1 Protein, Human, Recombinant (His Tag)

High Purity
> 98 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

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