Midkine Protein Overview: Sequence, Structure, Function and Protein Interaction

Midkine Protein Overview

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004).

Midkine protein family

Belongs to the pleiotrophin family.

Midkine protein name

Recommended name
Midkine
Short name
MK||ARAP
Aliases
FLJ27379, MK
Alternative name
Amphiregulin-associated protein Midgestation and kidney protein Neurite outgrowth-promoting factor 2 Neurite outgrowth-promoting protein

Midkine Protein Sequence

Species Human Midkine protein
Length 143
Mass (Da) 15585
Sequence Human Midkine protein sequence
Species Mouse Midkine protein
Length 140
Mass (Da) 15434
Sequence Mouse Midkine protein sequence
Species Rat Midkine protein
Length 140
Mass (Da) 15406
Sequence Rat Midkine protein sequence

Midkine Protein Molecular Weight & PI

Midkine precursor (MK) (Amphiregulin-associated protein) (ARAP) (Midgestation and kidney protein) (Neurite outgrowth-promoting factor 2) (Neurite outgrowth-promoting protein) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 21-143 Midkine.

Molecular weight (Da)

13420.51

Theoretical pI

9.78

Midkine Protein Structure

C-TERMINAL DOMAIN OF MIDKINE
Deposited
1999-03-16   Released:  1999-03-23
Deposition Author(s)
Iwasaki, W., Nagata, K., Hatanaka, H., Ogura, K., Inui, T., Kimura, T., Muramatsu, T., Yoshida, K., Tasumi, M., Inagaki, F.
Organism(s)
Homo sapiens
Experimental Data Snapshot
Method
SOLUTION NMR
1MKC From PDB

Human Midkine protein Secondary structure

Midkine Protein Interaction

Recombinant Midkine Protein Feature

Midkine Protein, Human, Recombinant

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA. Immobilized human MDK (Cat:10247-HNAB) at 10 μg/ml (100 μl/well) can bind mouse SDC4-Fc (Cat:50726-M02H) with a linear range of 0.16-1.25 μg/ml.

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