Midkine Protein Overview: Sequence, Structure, Function and Protein Interaction

Midkine Protein Overview

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004).

Midkine protein family

Belongs to the pleiotrophin family.

Midkine protein name

Recommended name
Short name
FLJ27379, MK
Alternative name
Amphiregulin-associated protein Midgestation and kidney protein Neurite outgrowth-promoting factor 2 Neurite outgrowth-promoting protein

Midkine Protein Sequence

Species Human Midkine protein
Length 143
Mass (Da) 15585
Sequence Human Midkine protein sequence
Species Mouse Midkine protein
Length 140
Mass (Da) 15434
Sequence Mouse Midkine protein sequence
Species Rat Midkine protein
Length 140
Mass (Da) 15406
Sequence Rat Midkine protein sequence

Midkine Protein Molecular Weight & PI

Midkine precursor (MK) (Amphiregulin-associated protein) (ARAP) (Midgestation and kidney protein) (Neurite outgrowth-promoting factor 2) (Neurite outgrowth-promoting protein) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 21-143 Midkine.

Molecular weight (Da)


Theoretical pI


Midkine Protein Structure

1999-03-16   Released:  1999-03-23
Deposition Author(s)
Iwasaki, W., Nagata, K., Hatanaka, H., Ogura, K., Inui, T., Kimura, T., Muramatsu, T., Yoshida, K., Tasumi, M., Inagaki, F.
Homo sapiens
Experimental Data Snapshot

Human Midkine protein Secondary structure

Midkine Protein Interaction

Recombinant Midkine Protein Feature

Midkine Protein, Human, Recombinant

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA. Immobilized human MDK (Cat:10247-HNAB) at 10 μg/ml (100 μl/well) can bind mouse SDC4-Fc (Cat:50726-M02H) with a linear range of 0.16-1.25 μg/ml.

Bulk Order of Recombinant Midkine Protein

Please Leave Us a Message if you have any questions regarding bulk price quote of our products on the website, our customer specialist will get back to you in 24 hours by email.

Custom Recombinant Protein Production Service Features

  • Over 10 years' experience for 6000+ recombinant proteins production.
  • One-stop service from gene synthesis and vector construction to protein expression and purification.
  • Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
  • Multiple purification systems (30+) to choose.
  • High R & D ability with over 1000 new proteins per year and quick problem-solving ability.
  • Close to 100 bioreactors with various volume between 2-1000 L to guarantee high-throughput and large-scale production.

Custom Recombinant Protein Production Service Advantages

  • High-efficiency expression vectors
  • High cell density culturing
  • Proprietary medium formulation
  • >6000 proteins expression and purification experience
  • High-efficiency expression vectors
  • High cell density culturing

Recombinant Protein Production Service Description

We offer one-stop services for recombinant protein production using our advanced protein expression platform technologies. Our full services include protein gene synthesis, protein codon optimization, protein expression vector design, large scale cell culture and fermentation, protein purification, and protein quality control testing. We offer significant cost saving advantages and record speed in protein expression and bulk production. We also have extensive experiences and expertise in handling various types of recombinant protein purification projects, with or without purification tags.