Hsp27/HSPB1 Protein Overview: Sequence, Structure, Function and Protein Interaction

Hsp27/HSPB1 Protein Overview

The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. Hickey et al. (1986) cloned a HeLa cell cDNA encoding HSP27. By screening a human genomic library with this HSP27 cDNA, Hickey et al. (1986) isolated the HSP27 genomic sequence. The deduced 199-amino acid HSP27 protein shows sequence similarity to mammalian alpha-crystallins (e.g., 123580). Approximately 20% of its residues are susceptible to phosphorylation. The HSP27 gene produced a 2.2-kb transcript in an in vitro transcription assay. Carper et al. (1990) cloned an HSP27 cDNA derived from heat-shocked human A549 lung carcinoma cells. The cDNA encodes a deduced 205-amino acid protein whose first 193 amino acids are identical to those of the predicted HSP27 protein reported by Hickey et al. (1986). Carper et al. (1990) suggested that the C-terminal differences of these deduced HSP27 proteins may be a result of a DNA sequencing artifact. PSEUDOGENESHickey et al. (1986) identified a processed HSP27 pseudogene.

Hsp27/HSPB1 protein family

Belongs to the small heat shock protein (HSP20) family.

Hsp27/HSPB1 protein name

Recommended name
Heat shock protein beta-1
Short name
HspB1||HSP 27||SRP27
Aliases
CMT2F, Hs.76067, Hsp25, HSP27, HSP28
Alternative name
28 kDa heat shock protein Estrogen-regulated 24 kDa protein Heat shock 27 kDa protein Stress-responsive protein 27

Hsp27/HSPB1 Gene family protein

Hsp27/HSPB1 Protein Sequence

Species Human Hsp27/HSPB1 protein
Length 205
Mass (Da) 22783
Sequence Human Hsp27/HSPB1 protein sequence
Species Mouse Hsp27/HSPB1 protein
Length 209
Mass (Da) 23014
Sequence Mouse Hsp27/HSPB1 protein sequence
Species Rat Hsp27/HSPB1 protein
Length 206
Mass (Da) 22893
Sequence Rat Hsp27/HSPB1 protein sequence

Hsp27/HSPB1 Protein Molecular Weight & PI

Heat shock protein beta-1 (HspB1) (28 kDa heat shock protein) (Estrogen-regulated 24 kDa protein) (Heat shock 27 kDa protein) (HSP 27) (Stress-responsive protein 27) (SRP27) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-205 Heat shock protein beta-1.

Molecular weight (Da)

22782.52

Theoretical pI

5.98

Hsp27/HSPB1 Protein Structure

Human Hsp27 core domain in complex with C-terminal peptide
Deposited
2013-09-03   Released:  2014-04-09
Deposition Author(s)
Laganowsky, A., Cascio, D., Sawaya, M.R., Eisenberg, D.
Organism(s)
Homo sapiens
Expression System
Escherichia coli BL21(DE3)
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.5990 Å
R-Value Free
0.266
R-Value Work
0.234
4MJH From PDB

Hsp27/HSPB1 Protein Interaction

Recombinant Hsp27/HSPB1 Protein Feature

Hsp27/HSPB1 Protein, Human, Recombinant (His Tag)

High Purity
> 97 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.

Recombinant Hsp27/HSPB1 protein citations

Title
Pan-amyloid oligomer specific scFv antibody attenuates memory deficits and brain amyloid burden in mice with Alzheimer's disease
Year
2014
Author
Zhao, M;Wang, SW;Wang, YJ;Zhang, R;Li, YN;Su, YJ;Zhou, WW;Yu, XL;Liu, RT;
Journal
Curr Alzheimer Res

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