Hsp27/HSPB1 Antibody Guideline: Preparation, Dilution and Applications

Hsp27/HSPB1 Antibody Overview

There are 10 Hsp27/HSPB1 antibodies which are validated in multiple tissues with various applications, including WB, ELISA, ELISA(Det), FCM, ICC/IF, ELISA(Cap), IP, IHC-P. There are 3 Hsp27/HSPB1 antibody for WB, 5 Hsp27/HSPB1 antibody for ELISA, 1 Hsp27/HSPB1 antibody for ELISA(Det), 5 Hsp27/HSPB1 antibody for FCM, 2 Hsp27/HSPB1 antibody for ICC/IF, 1 Hsp27/HSPB1 antibody for ELISA(Cap), 2 Hsp27/HSPB1 antibody for IP, 1 Hsp27/HSPB1 antibody for IHC-P. Among all these Hsp27/HSPB1 antibodies, there are 4 anti-Hsp27/HSPB1 mouse monoclonal antibodies , 4 anti-Hsp27/HSPB1 rabbit monoclonal antibodies , 2 anti-Hsp27/HSPB1 rabbit polyclonal antibodies . All the Hsp27/HSPB1 anbodies are produced in house and all are in stock. Hsp27/HSPB1 antibody customerized service is available.

Hsp27/HSPB1 gene family antibody

Hsp27/HSPB1 Antibody ELISA

Hsp27/HSPB1 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1-Lys 205). Total IgG was purified by Protein A affinity chromatography.

Hsp27/HSPB1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Hsp27/HSPB1 Antibody western blot

Hsp27/HSPB1 Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1 – Lys 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Hsp27/HSPB1 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

Hsp27/HSPB1 Antibody flow cytometry

Hsp27/HSPB1 Antibody immunohistochemistry

Hsp27/HSPB1 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1-Lys 205). HSP27 specific IgG was purified by Human HSP27 affinity chromatography.

Hsp27/HSPB1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:2500-1:10000
Notes  

Hsp27/HSPB1 Antibody immunoprecipitation

Hsp27/HSPB1 Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1-Lys 205) .

Hsp27/HSPB1 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

Hsp27/HSPB1 Antibody ICC / IF

Hsp27/HSPB1 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 / HSPB1 (rh HSP27 / HSPB1; Catalog#10351-H08E; NP_001532.1; Met1-Lys205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Hsp27/HSPB1 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes