ERK5/BMK1/MAPK7 Protein Overview: Sequence, Structure, Function and Protein Interaction

ERK5/BMK1/MAPK7 Protein Overview

Mitogen-activated protein kinases (MAPKs, or PRKMs) are activated by an upstream cascade of kinases in response to a wide variety of extracellular stimuli. Specific PRKM kinases (MAPKKs, or PRKMKs) have been shown to phosphorylate and activate specific PRKMs in a given signaling pathway. Using the yeast 2-hybrid system with mutant forms of PRKMK5 (602520) as baits, Zhou et al. (1995) identified a cDNA encoding MAPK7, called ERK5 by them. They demonstrated that MAPK7 interacts specifically with PRKMK5, but not with PRKMK1 (176872) or PRKMK2 (601263), suggesting that the PRKMK5/MAPK7 protein cascade is a novel signaling pathway. The 815-amino acid MAPK7 contains the conserved thr-glu-tyr activation motif of ERK-type MAPKs. MAPK7 has an uncharacteristic 400-amino acid C-terminal domain with sequences indicating that it may be targeted to the cytoskeleton. Zhou et al. (1995) found that full-length MAPK7 has no detectable kinase activity in vitro, whereas removal of the C terminus results in autophosphorylation, suggesting that the C terminus may play a role in regulating MAPK7. Northern blot analysis detected a 3.1-kb transcript in a number of human tissues, with the highest levels in heart and skeletal muscle. By degenerate PCR, Lee et al. (1995) isolated a cDNA encoding a PRKM which they named BMK1. BMK1 shows 98% amino acid identity to ERK5; they differ in a short span of residues at the N terminus. Lee et al. (1995) speculated that these kinases are derived from the same gene, possibly via differential mRNA splicing.

ERK5/BMK1/MAPK7 protein family

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

ERK5/BMK1/MAPK7 protein name

Recommended name
Mitogen-activated protein kinase 7
Aliases
BMK1, BMK1 kinase, ERK5, extracellular-signal-regulated kinase 5

ERK5/BMK1/MAPK7 Gene family protein

ERK5/BMK1/MAPK7 Protein Molecular Weight & PI

The parameters have been computed for the following feature

FT CHAIN 2-816 Mitogen-activated protein kinase 7.

Molecular weight (Da)

88255.28

Theoretical pI

5.60

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