EGFR Antibody Guideline: Preparation, Dilution and Applications

EGFR Antibody Overview

There are 31 EGFR antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, ICC/IF, IHC-P, WB, ELISA(Det), IHC-Fr, Neutralization, ELISA(Cap). There are 18 EGFR antibody for ELISA, 8 EGFR antibody for FCM, 3 EGFR antibody for ICC/IF, 3 EGFR antibody for IHC-P, 5 EGFR antibody for WB, 1 EGFR antibody for ELISA(Det), 1 EGFR antibody for IHC-Fr, 2 EGFR antibody for Neutralization, 1 EGFR antibody for ELISA(Cap). Among all these EGFR antibodies, there are 6 anti-EGFR mouse monoclonal antibodies , 12 anti-EGFR rabbit monoclonal antibodies , 13 anti-EGFR rabbit polyclonal antibodies . All the EGFR anbodies are produced in house and all are in stock. EGFR antibody customerized service is available.

EGFR gene family antibody

Approved symbol Common name
ERBB2 Her2/ERBB2 antibody
ERBB4 ErbB4 antibody
Approved symbol Common name
ERBB3 HER3/ERBB3 antibody

EGFR Antibody ELISA

EGFR antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / ErbB / HER1 (rh EGFR / ErbB / HER1; Catalog #10001-H08H; Met 1-Ser 645; NP_005219). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

EGFR antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

EGFR Antibody western blot

EGFR Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human EGFR / HER1 / ErbB1, and purified by antigen affinity chromatography.

EGFR Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

EGFR Antibody flow cytometry

EGFR Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645).

EGFR Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

EGFR Antibody immunohistochemistry

EGFR Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met 1-Ser 645).

EGFR Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

EGFR Antibody immunoprecipitation

EGFR Antibody ICC / IF

EGFR Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

EGFR Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:50-1:1000
Notes  

EGFR Antibody Neutralization

EGFR Antibody Neutralization Preparation

EGFR Antibody Neutralization Validated

Application Neutralization
Dilution The neutralization activity of EGFR Neutralizing Antibody is Measured by its ability to neutralize autocrine EGF induced proliferation in the human breast cancer MDA-MB-468 cell line. The Neutralization titer (IC50) is typically 0.05-0.2 μg/mL for 5000 cells/well.
Notes  

EGFR antibody citations

Title
Erbin interacts with c-Cbl and promotes tumourigenesis and tumour growth in colorectal cancer by preventing c-Cbl-mediated ubiquitination and down-regulation of EGFR
Year
2014
Author
Yao, S;Zheng, P;Wu, H;Song, LM;Ying, XF;Xing, C;Li, Y;Xiao, ZQ;Zhou, XN;Shen, T;Chen, L;Liu, YH;Lai, M;Mei, L;Gao, TM;Li, JM;
Journal
J. Pathol.
Title
Erbin interacts with c-Cbl and promotes tumourigenesis and tumour growth in colorectal cancer by preventing c-Cbl-mediated ubiquitination and down-regulation of EGFR
Year
2014
Author
Yao, S;Zheng, P;Wu, H;Song, LM;Ying, XF;Xing, C;Li, Y;Xiao, ZQ;Zhou, XN;Shen, T;Chen, L;Liu, YH;Lai, M;Mei, L;Gao, TM;Li, JM;
Journal
J. Pathol.
Title
Immunoselective Cellulose Nanospheres: A Versatile Platform for Nanotheranostics
Year
2014
Author
C Carrick, L Wågberg, PA Larsson
Journal
ACS Macro Letters
Title
In vitro effects and ex vivo binding of an EGFR-specific immunotoxin on rhabdomyosarcoma cells
Year
2014
Author
Niesen, J;Brehm, H;Stein, C;Berges, N;Pardo, A;Fischer, R;Ten Haaf, A;Gattenlöhner, S;Tur, MK;Barth, S;
Journal
J. Cancer Res. Clin. Oncol.
Title
The correlation between the expression of drug resistance gene and the recurrence of osteosarcoma
Year
2014
Author
WANG, J;LU, H;SUN, Y;YAO, Y;
Journal
TUMOR
Title
Enhancing tumor-specific intracellular delivering efficiency of cell-penetrating peptide by fusion with a peptide targeting to EGFR
Year
2015
Author
Nguyen, LT;Yang, XZ;Du, X;Wang, JW;Zhang, R;Zhao, J;Wang, FJ;Dong, Y;Li, PF;
Journal
Amino Acids
Title
Tuning the surface chemistry of gold nanoparticles to specifically image glioblastoma cells using surface-enhanced Raman spectroscopy
Year
2020
Author
Burgio, F;Piffaretti, D;Schmidt, F;Pieles, U;Reinert, M;Ritz, M;Saxer, S;
Journal
ACS Appl. Nano Mater.
Title
Live circulating tumour cells selection on digitized self-assembled cell array (Digi-saca) chip by in-parallel/in-situ image analysis, cell capture, and cultivation
Year
2020
Author
Goudar, V;Yeh, P;Wu, S;Chu, C;Lu, L;Yang, C;Chiou, T;Tseng, F;
Journal
Sensors and Actuators B: Chemical
Title
In Situ-Generated Multivalent Aptamer Network for Efficient Capture and Sensitive Electrochemical Detection of Circulating Tumor Cells in Whole Blood
Year
2020
Author
Yang, J;Li, X;Jiang, B;Yuan, R;Xiang, Y;
Journal
Anal. Chem.
Title
Increasing the antitumor efficacy of doxorubicin liposomes with coupling an anti-EGFR affibody in EGFR-expressing tumor models
Year
2020
Author
Jia, D;Yang, Y;Yuan, F;Fan, Q;Wang, F;Huang, Y;Song, H;Hu, P;Wang, R;Li, G;Liu, R;Li, J;
Journal
Int J Pharm
Title
Reusable on-plate immunoprecipitation method with covalently immobilized antibodies on a protein G covered microtiter plate
Year
2020
Author
Korodi, M;Rákosi, K;Baibarac, M;Fejer, SN;
Journal
J. Immunol. Methods
Title
Reusable on-plate immunoprecipitation method with covalently immobilized antibodies on a protein G covered microtiter plate
Year
2020
Author
Korodi, M;Rákosi, K;Baibarac, M;Fejer, SN;
Journal
J. Immunol. Methods