CD73 Antibody Guideline: Preparation, Dilution and Applications

CD73 Antibody Overview

There are 15 CD73 antibodies which are validated in multiple tissues with various applications, including WB, ELISA, FCM, IHC-P, ICC/IF, IP. There are 6 CD73 antibody for WB, 11 CD73 antibody for ELISA, 3 CD73 antibody for FCM, 3 CD73 antibody for IHC-P, 1 CD73 antibody for ICC/IF, 2 CD73 antibody for IP. Among all these CD73 antibodies, there are 4 anti-CD73 mouse monoclonal antibodies , 2 anti-CD73 rabbit monoclonal antibodies , 9 anti-CD73 rabbit polyclonal antibodies . All the CD73 anbodies are produced in house and all are in stock. CD73 antibody customerized service is available.

CD73 Antibody ELISA

CD73 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met 1-Lys 547). Total IgG was purified by Protein A affinity chromatography.

CD73 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD73 Antibody western blot

CD73 Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met 1-Lys 547). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD73 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD73 Antibody flow cytometry

CD73 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met1-Lys547). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD73 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD73 Antibody immunohistochemistry

CD73 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD73/NT5E (rM CD73/NT5E; Catalog#50231-M08H; NP_035981.1; Met1-Lys549). CD73/NT5E specific IgG was purified by Mouse CD73/NT5E affinity chromatography.

CD73 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

CD73 Antibody immunoprecipitation

CD73 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Rat CD73/NT5E (rR CD73/NT5E; Catalog#80335-R08H; Q66HL0; Met1-Lys549). CD73/NT5E specific IgG was purified by Rat CD73/NT5E affinity chromatography.

CD73 Antibody immunoprecipitation Validated

Application IP
Dilution 0.1-0.5 μL/mg of lysate
Notes  

CD73 Antibody ICC / IF

CD73 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met1-Lys547).

CD73 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes