CaMKII beta/CAMK2B Protein Overview: Sequence, Structure, Function and Protein Interaction

CaMKII beta/CAMK2B Protein Overview

Tombes and Krystal (1997) cloned a number of CAMK2B variants from several human tumor cell line and brain cDNA libraries. CAMK2B showed wide tissue and cell distribution, but 1 variant predominated in adult brain. The expression of other variants depended upon the tissue of origin and whether the source was normal or tumor tissue. By searching an EST database using rat Camk2 as query, followed by screening a brain cDNA library, Li et al. (2001) obtained a full-length cDNA encoding CAMK2B. The deduced protein contains 542 amino acids. A comparison of CAMK2B and CAMK2A (114078) detected no amino acid changes within the functional domains, including the ATP-binding site, the calmodulin-binding domain, and autophosphorylation sites. Northern blot analysis detected a major 3.9-kb transcript in adult and fetal brain; expression was slightly lower in fetal brain. Using PCR primers designed to identify alternatively spliced isoforms, Li et al. (2001) identified 3 CAMK2B isoforms in adult and fetal brain. Like other CAMK2 isoforms, the CAMK2B isoforms have an insertion or deletion of 11 to 39 amino acids within the central variable region. Wang et al. (2000) identified and cloned CAMK2B by searching an EST database using mouse Camk2b. They obtained a full-length cDNA by 5-prime RACE of a brain cDNA library. Wang et al. (2000) also identified 5 splice variants resulting from alternative splicing within 7 central variable domains, termed V1 to V7. The deduced proteins have calculated molecular masses between 49 and 60 kD. Transient transfection of 3 CAMK2B variants in HEK293 cells revealed that all 3 were cytosolic proteins.

CaMKII beta/CAMK2B protein family

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

CaMKII beta/CAMK2B protein name

Recommended name
Calcium/calmodulin-dependent protein kinase type II subunit beta
Short name
CaM kinase II subunit beta||CaMK-II subunit b
calcium / calmodulin-dependent protein kinase type II beta chain, CaM kinase II beta subunit, CAM2, CAMK2, CaM-kinase II beta chain, proline rich calmodulin-dependent protein kinase

CaMKII beta/CAMK2B Gene family protein

CaMKII beta/CAMK2B Protein Sequence

Species Human CaMKII beta/CAMK2B protein
Length 666
Mass (Da) 72678
Sequence Human CaMKII beta/CAMK2B protein sequence
Species Mouse CaMKII beta/CAMK2B protein
Length 542
Mass (Da) 60461
Sequence Mouse CaMKII beta/CAMK2B protein sequence
Species Rat CaMKII beta/CAMK2B protein
Length 542
Mass (Da) 60402
Sequence Rat CaMKII beta/CAMK2B protein sequence

CaMKII beta/CAMK2B Protein Molecular Weight & PI

Calcium/calmodulin-dependent protein kinase type II subunit beta (EC (CaM kinase II subunit beta) (CaMK-II subunit beta) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-666 Calcium/calmodulin-dependent protein

Molecular weight (Da)


Theoretical pI


CaMKII beta/CAMK2B Protein Structure

Crystal structure of human calcium/calmodulin-dependent protein kinase IIB isoform 1 (CAMK2B)
2007-11-28   Released:  2007-12-11
Deposition Author(s)
Filippakopoulos, P., Rellos, P., Niesen, F., Burgess, N., Bullock, A., Berridge, G., Pike, A.C.W., Ugochukwu, E., Pilka, E.S., von Delft, F., Arrowsmith, C.H., Edwards, A.M., Weigelt, J., Knapp, S., Structural Genomics Consortium (SGC)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
2.4000 Å
R-Value Free
R-Value Work

Human CaMKII beta/CAMK2B protein Secondary structure

CaMKII beta/CAMK2B Protein Interaction

Recombinant CaMKII beta/CAMK2B Protein Feature

CaMKII beta/CAMK2B Protein, Human, Recombinant (His & GST Tag)

High Purity
> 60 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
The specific activity was determined to be 300 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

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