CALML3 Antibody Guideline: Preparation, Dilution and Applications

CALML3 Antibody Overview

There are 5 CALML3 antibodies which are validated in multiple tissues with various applications, including ELISA, FCM, ICC/IF, WB, IHC-P, IP. There are 4 CALML3 antibody for ELISA, 1 CALML3 antibody for FCM, 1 CALML3 antibody for ICC/IF, 1 CALML3 antibody for WB, 1 CALML3 antibody for IHC-P, 1 CALML3 antibody for IP. Among all these CALML3 antibodies, there are 2 anti-CALML3 mouse monoclonal antibodies , 1 anti-CALML3 rabbit monoclonal antibodies , 2 anti-CALML3 rabbit polyclonal antibodies . All the CALML3 anbodies are produced in house and all are in stock. CALML3 antibody customerized service is available.

CALML3 Antibody ELISA

CALML3 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CALML3 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CALML3 Antibody western blot

CALML3 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). CALML3 specific IgG was purified by Human CALML3 affinity chromatography.

CALML3 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

CALML3 Antibody flow cytometry

CALML3 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CALML3 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CALML3 Antibody immunohistochemistry

CALML3 Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). CALML3 specific IgG was purified by Human CALML3 affinity chromatography.

CALML3 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

CALML3 Antibody immunoprecipitation

CALML3 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). CALML3 specific IgG was purified by Human CALML3 affinity chromatography.

CALML3 Antibody immunoprecipitation Validated

Application IP
Dilution 4-6 μL/mg of lysate
Notes  

CALML3 Antibody ICC / IF

CALML3 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CALML3 (rh CALML3; Catalog#12480-H07E; P27482; Met1-Lys149). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CALML3 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes