14-3-3 epsilon Antibody Guideline: Preparation, Dilution and Applications

14-3-3 epsilon Antibody Overview

There are 3 14-3-3 epsilon antibodies which are validated in multiple tissues with various applications, including ELISA, WB, ICC/IF, IP. There are 2 14-3-3 epsilon antibody for ELISA, 2 14-3-3 epsilon antibody for WB, 2 14-3-3 epsilon antibody for ICC/IF, 2 14-3-3 epsilon antibody for IP. Among all these 14-3-3 epsilon antibodies, there are 1 anti-14-3-3 epsilon mouse monoclonal antibodies , 2 anti-14-3-3 epsilon rabbit polyclonal antibodies . All the 14-3-3 epsilon anbodies are produced in house and all are in stock. 14-3-3 epsilon antibody customerized service is available.

14-3-3 epsilon gene family antibody

Approved symbol Common name
SFN 14-3-3 sigma antibody
YWHAH 14-3-3 eta/YWHAH antibody
Approved symbol Common name
YWHAB 14-3-3 beta antibody
YWHAQ 14‑3‑3/YWHAQ antibody

14-3-3 epsilon Antibody ELISA

14-3-3 epsilon antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 epsilon / YWHAE (rh 14-3-3 epsilon / YWHAE; Catalog#10842-HNCE; NP_006752.1; Met1-Gln255). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

14-3-3 epsilon antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

14-3-3 epsilon Antibody western blot

14-3-3 epsilon Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human 14-3-3 epsilon/YWHAE (rh 14-3-3 epsilon/YWHAE; Catalog#10842-HNCE; NP_006752.1; Met1-Gln255). 14-3-3 epsilon/YWHAE specific IgG was purified by Human 14-3-3 epsilon/YWHAE affinity chromatography.

14-3-3 epsilon Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

14-3-3 epsilon Antibody immunoprecipitation

14-3-3 epsilon Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human 14-3-3 epsilon/YWHAE (rh 14-3-3 epsilon/YWHAE; Catalog#10842-HNCE; NP_006752.1; Met1-Gln255). 14-3-3 epsilon/YWHAE specific IgG was purified by Human 14-3-3 epsilon/YWHAE affinity chromatography.

14-3-3 epsilon Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

14-3-3 epsilon Antibody ICC / IF

14-3-3 epsilon Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Human 14-3-3 epsilon/YWHAE (rh 14-3-3 epsilon/YWHAE; Catalog#10842-HNCE; NP_006752.1; Met1-Gln255). 14-3-3 epsilon/YWHAE specific IgG was purified by Human 14-3-3 epsilon/YWHAE affinity chromatography.

14-3-3 epsilon Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes