Interferon Gamma Protein, Rat, Recombinant (hFc Tag): Product Information
> 92 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 1-5 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized rat IFNG-Fc (cat:80234-R02H) at 10 μg/ml (100 μl/well) can bind biotinylated rat IFNGR-Fc (Cat:80182-R02H). The EC50 of biotinylated rat IFNGR-Fc (Cat:80182-R02H) is 20.1-46.9 ng/ml.
A DNA sequence encoding the rat IFNG (P01581) (Met 1-Cys 156) was fused with the Fc region of human IgG1 at the C-terminus.
The secreted recombinant rat IFNG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 375 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the rat IFNG/Fc monomer is approximately 52 kDa in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Interferon Gamma Protein, Rat, Recombinant (hFc Tag): Images
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 1-5 ng/mL.
Interferon Gamma Background Information
IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
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