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TNFR2/TNFRSF1B Protein, Human, Recombinant (His & hFc Tag)

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TNFR2/TNFRSF1B Protein, Human, Recombinant (His & hFc Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 4-16 ng/ml in the presence of 0.25 ng/mL recombinant human TNF-α.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Asp 257) of human TNFRII (NP_001057.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Leu 23
Molecule Mass
The recombinant human TNFRII/Fc is a disulfide-linked homodimer. The reduced monomer consists of 482 amino acids and has a predicted molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII/Fc monomer is approximately 75 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNFR2/TNFRSF1B Protein, Human, Recombinant (His & hFc Tag): Images

Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 4-16 ng/ml in the presence of 0.25 ng/mL recombinant human TNF-α.

TNFR2/TNFRSF1B Protein, Human, Recombinant (His & hFc Tag): Alternative Names

CD120b Protein, Human; p75 Protein, Human; p75TNFR Protein, Human; TBPII Protein, Human; TNF-R-II Protein, Human; TNF-R75 Protein, Human; TNFBR Protein, Human; TNFR1B Protein, Human; TNFR2 Protein, Human; TNFR80 Protein, Human

TNFR2/TNFRSF1B Background Information

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD12b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD12b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD12b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD12b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Full Name
tumor necrosis factor receptor superfamily, member 1b
References
  • Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
  • Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
  • Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
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