SMAC Protein, Human, Recombinant (His Tag)

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SMAC Protein, Human, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human SMAC-His (Cat:10339-H08E)at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI (Cat:10606-H17E) with a linear range of 0.125-1.0 μg/ml.
Protein Construction
A DNA sequence encoding the mitochondrial-located form of human Diablo (NP_063940.1) (Ala 56-Asp 239) was expressed with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human Diablo/SMAC consists of 191 amino acids and migrates as polypeptide of 22 kDa as estimated by SDS-PAGE under reduced and non-reduced conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SMAC Protein, Human, Recombinant (His Tag): Images

Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His (Cat: 10339-H08E)at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI (Cat: 10606-H17E) with a linear range of 0.125-1.0 μg/ml.

SMAC Protein, Human, Recombinant (His Tag): Synonyms

DFNA64 Protein, Human; SMAC Protein, Human

SMAC Background Information

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.
Full Name
diablo, IAP-binding mitochondrial protein
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • TNF Signaling
    TNF Signaling
References
  • Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.
  • Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.
  • Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.
  • cIAP1 attenuates shear stress-induced hBMSC apoptosis for tissue-engineered blood vessels through the inhibition of the mitochondrial apoptosis pathway
    Author
    Zhao, D;Sun, Y;Wei, X;Liang, H;Zhao, L;Dong, X;Chen, H;Chen, W;Yang, J;Wang, X;Gao, F;Yi, W;
    Year
    2015
    Journal
    Life Sci.
    Application
    cell-based
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