SIRP alpha Protein, Human, Recombinant (ECD, His Tag): Product Information
> 98 % as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (Cat:12283-H02H), The EC50 of human CD47-Fc (Cat:12283-H02H) is 10.1-23.5 ng/ml.
A DNA sequence encoding the human SIRPA (NP_542970.1, with natural variant Gly 75 Ala) extracellular domain (Met1-Arg370) was expressed fused with a polyhistidine tag at the C-terminus.
The recombinant human SIRPA consists of 351 amino acids and predictes a molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human SIRPA is approximately 45-55 kDa due to glycosylation.
Lyophilized from sterile PBS, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SIRP alpha Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (Cat: 12283-H02H), The EC50 of human CD47-Fc (Cat: 12283-H02H) is 10.1-23.5 ng/ml.
SIRP alpha Protein, Human, Recombinant (ECD, His Tag): Synonyms
Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
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