IL17 Protein, Human, Recombinant
Cat: 12047-HNAE
IL17 Protein, Human, Recombinant: Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50for this effect is 0.5-2.5 ng/mL.
Protein Construction
A DNA sequence encoding the human IL17A (Q16552) (Ile20-Ala155) was expressed, with a N-terminal Met.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human IL17A consists of 137 amino acids and predicts a molecular mass of 15.7 KDa.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL17 Protein, Human, Recombinant: Images
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50for this effect is 0.5-2.5 ng/mL.
IL17 Protein, Human, Recombinant: Synonyms
CTLA-8 Protein, Human; CTLA8 Protein, Human; IL-17 Protein, Human; IL-17A Protein, Human; IL17 Protein, Human
IL17 Background Information
IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.
Full Name
interleukin 17A
Research Areas
Related Pathways
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IL12 signaling pathway -
IL17 signaling pathway
References
- Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7.
- Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52.
- Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52.
- Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.
Mouse IL17 Proteins
Cynomolgus IL17 Proteins
Canine IL17 Proteins
Rabbit IL17 Proteins
Rhesus IL17 Proteins
Marmoset IL17 Proteins
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Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 SignallingAuthorZhang, H;Ju, B;Zhang, X;Zhu, Y;Nie, Y;Xu, Y;Lei, Q;Year2016JournalBasic & Clinical Phamacology & ToxocologyApplicationcell based (culture)
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Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in PsoriasisAuthorLei, H;Li, X;Jing, B;Xu, H;Wu, Y;Year2017JournalPLoS ONEApplicationcell-based
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Computational Design of Epitope-Specific Functional AntibodiesAuthorNimrod, G;Fischman, S;Austin, M;Herman, A;Keyes, F;Leiderman, O;Hargreaves, D;Strajbl, M;Breed, J;Klompus, S;Minton, K;Spooner, J;Buchanan, A;Vaughan, TJ;Ofran, Y;Year2018JournalCell RepApplicationbinding and ELISA
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