IL17 Protein, Human, Recombinant (His Tag)

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IL17 Protein, Human, Recombinant (His Tag): Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized Human IL17 His (Cat:12047-H07Y) at 2 μg/ml (100 μl/well) can bind Human IL17RA His & hFc (Cat:10895-H03H), the EC50 of Human IL17RA His & hFc is 30-180 ng/mL.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The EC50 for this effect is typically 5-25 ng/ml.
Protein Construction
A DNA sequence encoding human IL17A (Q16552) (Gly24-Ala155) was expressed with a polyhistidine tag at the N-terminus.
Accession#
Expressed Host
Yeast
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human human IL17A consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 17 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL17 Protein, Human, Recombinant (His Tag): Images

Measured by its binding ability in a functional ELISA. Immobilized Human IL17 His (Cat:12047-H07Y) at 2 μg/ml (100 μl/well) can bind Human IL17RA His & hFc (Cat:10895-H03H), the EC50 of Human IL17RA His & hFc is 30-180 ng/mL.

IL17 Protein, Human, Recombinant (His Tag): Synonyms

CTLA-8 Protein, Human; CTLA8 Protein, Human; IL-17 Protein, Human; IL-17A Protein, Human; IL17 Protein, Human

IL17 Background Information

IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.
Full Name
interleukin 17A
Research Areas
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7.
  • Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52.
  • Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52.
  • Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.
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