IL17 Protein, Human, Recombinant (His Tag): Product Information
> 90 % as determined by SDS-PAGE
Please contact us for more information.
1. Measured by its binding ability in a functional ELISA. Immobilized Human IL17 His (Cat:12047-H07Y) at 2 μg/ml (100 μl/well) can bind Human IL17RA His & hFc (Cat:10895-H03H), the EC50 of Human IL17RA His & hFc is 30-180 ng/mL. 2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The EC50 for this effect is typically 5-25 ng/ml.
A DNA sequence encoding human IL17A (Q16552) (Gly24-Ala155) was expressed with a polyhistidine tag at the N-terminus.
The recombinant human human IL17A consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 17 KDa band in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Measured by its binding ability in a functional ELISA. Immobilized Human IL17 His (Cat:12047-H07Y) at 2 μg/ml (100 μl/well) can bind Human IL17RA His & hFc (Cat:10895-H03H), the EC50 of Human IL17RA His & hFc is 30-180 ng/mL.
IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.
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