EGFR Protein, Human, Recombinant (ECD, His Tag)
Cat: 10001-H08H
EGFR Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc (Cat: 10605-H01H) at 2 μg/ml (100 μl/well) can bind Human EGFR His (Cat: 10001-H08H), the EC50 of Human EGFR His is 100-500 ng/mL.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Leu 25
Molecule Mass
The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EGFR Protein, Human, Recombinant (ECD, His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc (Cat: 10605-H01H) at 2 μg/ml (100 μl/well) can bind Human EGFR His (Cat: 10001-H08H), the EC50 of Human EGFR His is 100-500 ng/mL.
EGFR Protein, Human, Recombinant (ECD, His Tag): Synonyms
ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human
EGFR Background Information
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Full Name
epidermal growth factor receptor
Research Areas
Related Pathways
-
EGFR Signaling Pathway -
Jak-Stat Signaling Pathway
References
- Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
- Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
- Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
Human EGFR Proteins
Rat EGFR Proteins
Canine EGFR Proteins
-
Phage-derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGFAuthorZhang, J;Li, H;Wang, X;Qi, H;Miao, X;Zhang, T;Chen, G;Wang, M;Year2012JournalBiotechnol. Prog.Applicationbinding (negative control)
-
Phage‐derived fully human antibody scFv fragment directed against human vascular endothelial growth factor receptor 2 blocked its interaction with VEGFAuthorJ Zhang, H Li, X Wang, H Qi, X Miao…Year2012JournalBiotechnol. ProgApplicationnegative control in the test of the specificity of scFv antibodies
-
Erbin interacts with c-Cbl and promotes tumourigenesis and tumour growth in colorectal cancer by preventing c-Cbl-mediated ubiquitination and down-regulation of EGFRAuthorYao, S;Zheng, P;Wu, H;Song, LM;Ying, XF;Xing, C;Li, Y;Xiao, ZQ;Zhou, XN;Shen, T;Chen, L;Liu, YH;Lai, M;Mei, L;Gao, TM;Li, JM;Year2014JournalJ. Pathol.
-
VHH characterization.Recombinant VHHs: Production, characterization and affinityAuthorChabrol, E;Stojko, J;Nicolas, A;Botzanowski, T;Fould, B;Antoine, M;Cianférani, S;Ferry, G;Boutin, J;Year2019JournalAnalytical Biochemistry
-
Enzymes Photo-Cross-Linked to Live Cell Receptors Retain Activity and EGFR Inhibition after Both Internalization and RecyclingAuthorRoy, S;Brasino, M;Beirne, JM;Harguindey, A;Chapnick, DA;Liu, X;Cha, JN;Goodwin, AP;Year2019JournalBioconjug. Chem.Applicationcell-based
-
Effects of viral promoters, the Woodchuck hepatitis post-transcriptional regulatory element, and weakened antibiotic resistance markers on transgene expression in Chinese hamster ovary cellsAuthorWang, W;Guo, X;Chen, S;Wang, T;Wang, F;Li, Q;Wang, Y;Year2020JournalProcess BiochemistryApplicationWB; Elisa
If you couldn't find the specific citation, you can:
