CD48 Protein, Human, Recombinant (His Tag)

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CD48 Protein, Human, Recombinant (His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured by its binding ability in a functional ELISA . Immobilized human CD48 at 10 μg/ml (100 μl/well) can bind recombinant human 2B4 / CD244 with a linear range of 0.004-0.4 μg/ml.
Protein Construction
A DNA sequence encoding the human CD48 (NP_001769.2) (Met 1-Ser 220) with a C-terminal polyhistidine tag was expressed.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 27
Molecule Mass
The secreted recombinant human CD48 comprises 205 amino acids with a predicted molecular mass of 23.8 kDa. As a result of glycosylation, rhCD48 migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD48 Protein, Human, Recombinant (His Tag): Images

Measured by its binding ability in a functional ELISA. Immobilized human CD48 at 10 μg/ml (100 μl/well) can bind recombinant human 2B4 / CD244 with a linear range of 0.004-0.4 μg/ml.

CD48 Protein, Human, Recombinant (His Tag): Synonyms

BCM1 Protein, Human; BLAST Protein, Human; Blast-1 Protein, Human; BLAST1 Protein, Human; hCD48 Protein, Human; mCD48 Protein, Human; MEM-102 Protein, Human; SLAMF2 Protein, Human

CD48 Background Information

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Full Name
CD48 molecule
Research Areas
References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
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