TNF-alpha Matched ELISA Antibody Pair Set,Human

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TNF-alpha Matched ELISA Antibody Pair Set,Human General Information

Product name
TNF-alpha Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human TNF-alpha
Assay range
39.06-2500 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10602-MM01)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human TNF-alpha / TNFA / TNFSF2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in Dilution Buffer before use. (Catalog: # 10602-MM08)
3. Standard Each vial contains 55 ng of recombinant Human TNF-alpha / TNFA / TNFSF2. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2500 pg/mL is recommended.
Product overview
This TNF-alpha Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human TNF-alpha . It contains Human TNF-alpha capture antibody, Human TNF-alpha detector antibody (HRP) and a highly purified E. coli-expressed recombinant Human TNF-alpha protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

TNF-alpha Matched ELISA Antibody Pair Set,Human Images

TNF-alpha Matched ELISA Antibody Pair Set,Human: Synonyms

DIF Matched ELISA Antibody Pair Set, Human; TNF-alpha Matched ELISA Antibody Pair Set, Human; TNFA Matched ELISA Antibody Pair Set, Human; TNFSF2 Matched ELISA Antibody Pair Set, Human

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Full Name
tumor necrosis factor
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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    Author
    Hamed, HO;Ahmed, SR;Alzolibani, A;Kamal, MM;Mostafa, MS;Gamal, RM;Atallah, DA;Abd-El-Aall, DE;
    Year
    2013
    Journal
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    Application
    ELISA
  • Rheumatoid arthritis-associated microRNA-155 targets SOCS1 and upregulates TNF-α and IL-1β in PBMCs
    Author
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    Journal
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    Application
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