TNF-alpha Matched ELISA Antibody Pair Set,Mouse

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TNF-alpha Matched ELISA Antibody Pair Set,Mouse General Information

Product name
TNF-alpha Matched ELISA Antibody Pair Set,Mouse
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Mouse TNF-alpha
Assay range
31.25-2000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50349-R023)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.2 μg/mL in Dilution Buffer before use.
3. Standard Each vial contains 87 ng of recombinant Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2000 pg/mL is recommended.
Product overview
This TNF-alpha Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse TNF-alpha . It contains Mouse TNF-alpha capture antibody, Mouse TNF-alpha detector antibody (HRP) and a highly purified E. coli-expressed recombinant Mouse TNF-alpha protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

TNF-alpha Matched ELISA Antibody Pair Set,Mouse Images

TNF-alpha Matched ELISA Antibody Pair Set,Mouse: Synonyms

DIF Matched ELISA Antibody Pair Set, Mouse; TNF-a Matched ELISA Antibody Pair Set, Mouse; TNF-alpha Matched ELISA Antibody Pair Set, Mouse; Tnfa Matched ELISA Antibody Pair Set, Mouse; TNFalpha Matched ELISA Antibody Pair Set, Mouse; Tnfsf1a Matched ELISA Antibody Pair Set, Mouse; TNFSF2 Matched ELISA Antibody Pair Set, Mouse

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Full Name
tumor necrosis factor
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • The effect of exposure time and concentration of airborne PM2.5 on lung injury in mice: A transcriptome analysis
    Author
    Wang, H;Shen, X;Liu, J;Wu, C;Gao, J;Zhang, Z;Zhang, F;Ding, W;Lu, Z;
    Year
    2019
    Journal
    Redox Biology
    Application
    ELISA
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