Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set

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Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set General Information

Product name
Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Influenza Hemagglutinin/HA
Assay range
62.5-4000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of rabbit anti-Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 0.5 μg/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 110 ng of recombinant Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
Product overview
This Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of Influenza Hemagglutinin/HA . It contains Influenza Hemagglutinin/HA capture antibody, Influenza Hemagglutinin/HA detector antibody (HRP) and a highly purified Baculovirus-Insect-expressed recombinant Influenza Hemagglutinin/HA protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set Images

Hemagglutinin/HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
  • Recombinant hemagglutinin produced from Chinese Hamster Ovary (CHO) stable cell clones and a PELC/CpG combination adjuvant for H7N9 subunit vaccine development
    Author
    Chen, TH;Liu, WC;Chen, IC;Liu, CC;Huang, MH;Jan, JT;Wu, SC;
    Year
    2019
    Journal
    Vaccine
    Application
    ELISA
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