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DKK3 Matched ELISA Antibody Pair Set,Mouse

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DKK3 Matched ELISA Antibody Pair Set,Mouse General Information

Product name
DKK3 Matched ELISA Antibody Pair Set,Mouse
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Mouse DKK3
Assay range
15.63-1000 pg/mL
Materials provided
1. Capture Antibody 0.5 mg/mL of rabbit anti-mouse DKK3 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 50247-R019)
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse DKK3 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 15 ng of recombinant mouse DKK3. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Product overview
This DKK3 Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse DKK3 . It contains Mouse DKK3 capture antibody, Mouse DKK3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse DKK3 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

DKK3 Matched ELISA Antibody Pair Set,Mouse Images

DKK3 Matched ELISA Antibody Pair Set,Mouse: Alternative Names

AW061014 Matched ELISA Antibody Pair Set, Mouse; C87148 Matched ELISA Antibody Pair Set, Mouse

DKK3 Background Information

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.
Full Name
dickkopf WNT signaling pathway inhibitor 3
References
  • Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.
  • Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.
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