CD86 Matched ELISA Antibody Pair Set,Rat General Information
CD86 Matched ELISA Antibody Pair Set,Rat
Solid Phase Sandwich ELISA
Quantitative determination of Rat CD86
1. Capture Antibody 0.4 mg/mL of mouse anti-rat CD86 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 80025-MM12) 2. Detection Antibody 0.25 mg/mL mouse anti-rat CD86 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0. 5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 80025-MM10) 3. Standard Each vial contains 19 ng of recombinant rat CD86. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.3 ng/mL is recommended.
This CD86 Matched ELISA Antibody Pair Set,Rat is a solid phase sandwich ELISA for quantitative determination of Rat CD86 . It contains Rat CD86 capture antibody, Rat CD86 detector antibody
and a highly purified
recombinant Rat CD86 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD86 Matched ELISA Antibody Pair Set,Rat Images
CD86 Background Information
CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
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