B4GALT1 Matched ELISA Antibody Pair Set,Human

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B4GALT1 Matched ELISA Antibody Pair Set,Human General Information

Product name
B4GALT1 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human B4GALT1
Assay range
156.25-10000 pg/mL
Materials provided
1. Capture Antibody 1.0 mg/mL of rabbit anti-B4GALT1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 11220-R001)
2. Detection Antibody 0.5 mg/mL rabbit anti-B4GALT1 polyoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 300 ng of recombinant B4GALT1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended.
Product overview
This B4GALT1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human B4GALT1 . It contains Human B4GALT1 capture antibody, Human B4GALT1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human B4GALT1 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

B4GALT1 Matched ELISA Antibody Pair Set,Human Images

B4GALT1 Matched ELISA Antibody Pair Set,Human: Synonyms

B4GAL-T1 Matched ELISA Antibody Pair Set, Human; beta4Gal-T1 Matched ELISA Antibody Pair Set, Human; CDG2D Matched ELISA Antibody Pair Set, Human; GGTB2 Matched ELISA Antibody Pair Set, Human; GT1 Matched ELISA Antibody Pair Set, Human; GTB Matched ELISA Antibody Pair Set, Human

B4GALT1 Background Information

Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.
Full Name
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
References
  • Hennet T. (2002) The galactosyltransferase family. Cellular and Molecular Life Sciences. 59(7): 1081-95.
  • Landers EA, et al. (2009) Porcine 1, 4-Galactosyltransferase-I Sequence and Expression. Reproduction in Domestic Animals. 44(2): 228-34.
  • Amado M, et al. (2000) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1473 (1): 35-53.
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