M-CSF Protein

M-CSF Protein Overview

M-CSF reagents

CSF1 is a cytokine required for differentiation of monocyte lineage cells (e.g., tissue macrophages, osteoclasts, and microglia) during development (Kubota et al., 2009).

M-CSF protein name

Recommended name
Macrophage colony-stimulating factor 1
Short name
CSF-1||M-CSF||MCSF
Aliases
macrophage colony stimulating factor 1, MCSF, M-CSF, MGC31930
Alternative name
Lanimostim Processed macrophage colony-stimulating factor 1

M-CSF Protein Sequence

Species Human M-CSF protein
Length 554
Mass (Da) 60179
Sequence Human M-CSF protein sequence
Species Mouse M-CSF protein
Length 552
Mass (Da) 60649
Sequence Mouse M-CSF protein sequence
Species Rat M-CSF protein
Length 566
Mass (Da) 62187
Sequence Rat M-CSF protein sequence

M-CSF Protein Molecular Weight & PI

Macrophage colony-stimulating factor 1 precursor (CSF-1) (M-CSF) (MCSF) (Lanimostim) [Contains: Processed macrophage colony-stimulating factor 1] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 33-554 Macrophage colony-stimulating factor 1.

Molecular weight (Da)

56926.52

Theoretical pI

5.07

M-CSF Protein Structure

Crystal structure of the human Colony-Stimulating Factor 1 (hCSF-1) cytokine
Deposited
2011-10-31   Released:  2012-08-22
Deposition Author(s)
Elegheert, J., Savvides, S.N.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.7500 Å
R-Value Free
0.190
R-Value Work
0.137
3UF2 From PDB

Human M-CSF protein Secondary structure

M-CSF Protein Interaction

Recombinant M-CSF Protein Feature

M-CSF Protein, Human, Recombinant

High Purity
> 85 % as determined by SDS-PAGE.
Low Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
High Activity
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.6-6.3 ng/mL.

Recombinant M-CSF protein citations

Title
A potent in vivo anti-tumor efficacy of novel recombinant type I interferon
Year
2016
Author
Zhang, K;Yin, XF;Yang, YQ;Li, HL;Xu, YN;Chen, LY;Liu, XJ;Yuan, SJ;Fang, XL;Xiao, J;Wu, S;Xu, H;Chu, L;Katlinski, KV;Katlinskaya, YV;Guo, RB;Wei, GW;Wang, DC;Liu, X;Fuchs, SY;
Journal
Clin. Cancer Res.
Title
A potent in vivo anti-tumor efficacy of novel recombinant type I interferon
Year
2016
Author
Zhang, K;Yin, XF;Yang, YQ;Li, HL;Xu, YN;Chen, LY;Liu, XJ;Yuan, SJ;Fang, XL;Xiao, J;Wu, S;Xu, H;Chu, L;Katlinski, KV;Katlinskaya, YV;Guo, RB;Wei, GW;Wang, DC;Liu, X;Fuchs, SY;
Journal
Clin. Cancer Res.
Title
Automating hESC differentiation with 3D printing and legacy liquid handling solutions
Year
2016
Author
Zluhan, E;Kelly, K;LeClair, N;Wortel, D;Moody, K;
Journal
MethodsX
Title
TIMAP repression by TGFβ and HDAC3-associated Smad signaling regulates macrophage M2 phenotypic phagocytosis
Year
2016
Author
Yang, J;Yin, S;Bi, F;Liu, L;Qin, T;Wang, H;Cao, W;
Journal
J. Mol. Med.
Title
EM, an Engineered Endostatin with High ATPase Activity, Inhibits the Recruitment and Alternative Activation of Macrophages in Non-small Cell Lung Cancer
Year
2017
Author
Xu, M;Zhang, S;Jia, L;Wang, S;Liu, J;Ma, X;Wang, C;Fu, Y;Luo, Y;
Journal
Frontiers in Pharmacology
Title
IL-17A promotes osteoclast formation via inducing RANKL in synovial cells
Year
2017
Author
Wang, J;Zhou, F;Zhao, X;Pang, Y;Zhao, S;
Journal
Int J Clin Exp Pathol
Title
Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB
Year
2017
Author
Gao, SY;Zheng, GS;Wang, L;Liang, YJ;Zhang, SE;Lao, XM;Li, K;Liao, GQ;
Journal
PLoS ONE
Title
Crosstalk between hepatic tumor cells and macrophages via Wnt/β-catenin signaling promotes M2-like macrophage polarization and reinforces tumor malignant behaviors
Year
2018
Author
Yang, Y;Ye, YC;Chen, Y;Zhao, JL;Gao, CC;Han, H;Liu, WC;Qin, HY;
Journal
Cell Death Dis

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