Human YKL-40/CHI3L1 HEK293 Overexpression Lysate

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Human YKL-40/CHI3L1 HEK293 Overexpression Lysate: Product Information

Product Description
This Human YKL-40/CHI3L1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of YKL-40/CHI3L1 protein (Cat: 11227-H08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human CHI3L1 (NP_001267.2) (Met 1-Thr 383) was fused with a polyhistidine tag at the C-terminus.
Molecule Mass
The recombinant human CHI3L1 consists of 373 amino acids and has a predicted molecular mass of 42 kDa as estimated in SDS-PAGE under reducing conditions.

Human YKL-40/CHI3L1 HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human YKL-40/CHI3L1 HEK293 Overexpression Lysate: Alternative Names

Human ASRT7 Overexpression Lysate; Human CGP-39 Overexpression Lysate; Human DKFZp686N19119 Overexpression Lysate; Human FLJ38139 Overexpression Lysate; Human GP-39 Overexpression Lysate; Human GP39 Overexpression Lysate; Human HC-gp39 Overexpression Lysate; Human hCGP-39 Overexpression Lysate; Human HCGP-3P Overexpression Lysate; Human YKL-40 Overexpression Lysate; Human YKL40 Overexpression Lysate; Human YYL-40 Overexpression Lysate

YKL-40/CHI3L1 Background Information

Chitinase-3-like protein 1 (CHI3L1) is a secreted heparin-binding glycoprotein whose expression is associated with vascular smooth muscle cell migration. CHI3L1 is expressed at high levels in postconfluent nodular VSMC cultures and at low levels in subconfluent proliferating cultures. CHI3L1 is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyto / macrophage markers, its expression is absent in monocytes and strong induced during late stages of human macrophage differentiation. Elevated levels of CHI3L1 are associated with disorders exhibiting increased connective tissue turnover, such as rheum atoid, arthritis, osteoarthritis, scleroderma, and cirrhosis of liver, but is produced in cartilage from old donors or patiens with osteoarthritis. CHI3L1 is abnormally expressed in the hippocampus of subjects with schizophrenia and may be involved in the cellular response to various environmental events that are reported to increase the risk of schizophrenia.
Full Name
chitinase 3 like 1
References
  • Zhao XZH, et al. (2007) Functional Variants in the Promoter Region of Chitinase 3-Like 1 (CHI3L1) and Susceptibility to Schizophrenia.The American Journal of Human Genetics. 80 (1): 12-18.
  • Rehli M, et al. (2003) Transcriptional Regulation of CHI3L1, a Marker Gene for Late Stages of Macrophage Differentiation . The Journal of Biological Chemistry. 278: 44058-67.
  • Nishikawa KC, et al. (2003) gp38k (CHI3L1) is a novel adhesion and migration factor for vascular cells. Experimental Cell Research. 287 (1): 79-87
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