Human VWC2 HEK293 Overexpression Lysate: Product Information
This Human VWC2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of VWC2 protein (Cat: 12071-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human VWC2 (NP_940972.2) (Met 1-Met 325) was expressed, fused with a polyhistidine tag at the C-terminus.
The recombinant human VWC2 consists of 309 amino acids and predictes a molecular mass of 34.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh VWC2 is approximately 45 kDa due to glycosylation.
Human VWC2 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human VWC2 HEK293 Overexpression Lysate: Alternative Names
Human PSST739 Overexpression Lysate; Human UNQ739 Overexpression Lysate
VWC2 Background Information
Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein which contains twoVWFC domains. VWC2 / Brorin is a BMP antagonist which may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.
von Willebrand factor C domain containing 2
Koike,N. et al., 2007, J Biol Chem. 282 (21):15843-50.
Elis,S. et al., 2009,Mol Reprod Dev. 76 (11):1043-55.
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