Human ULBP2 HEK293 Overexpression Lysate

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Human ULBP2 HEK293 Overexpression Lysate: Product Information

Product Description
This Human ULBP2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ULBP2 protein (Cat: 12143-H02H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human ULBP2 (Q9BZM5) (Met 1-Ser 217),without the pro peptide, w was fused with the Fc region of human IgG1 at the C-terminus.
Molecule Mass
The recombinant human ULBP2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 433 amino acids and has a predicted molecular mass of 48.7 kDa. As a result of glycosylation, the apparent molecular mass of rhULBP2/Fc monomer is approximately 58 kDa in SDS-PAGE under reducing conditions.

Human ULBP2 HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human ULBP2 HEK293 Overexpression Lysate: Alternative Names

Human ALCAN-alpha Overexpression Lysate; Human N2DL2 Overexpression Lysate; Human NKG2DL2 Overexpression Lysate; Human RAET1H Overexpression Lysate; Human UNQ463 / PRO791 Overexpression Lysate

ULBP2 Background Information

NKG2D ligand 2, also known as N2DL-2, NKG2DL2, ALCAN-alpha, Retinoic acid early transcript 1H, UL16-binding protein 2, ULBP2 and N2DL2, is cell membrane protein which belongs to theMHC class I family. ULBP2 / N2DL-2 is expressed in various types of cancer cell lines and in the fetus, but not in normal tissues. ULBP2 / N2DL-2 is a ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway.
Full Name
UL16 binding protein 2
References
  • Cosman D. et al., 2001,Immunity 14:123-133
  • Cerwenka A., et al., 2003, Tissue Antigens 61:335-343
  • Chang,Y.T. et al., 2011, PLoS One. 6 (5):e20029
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