Human Tomoregulin-1 HEK293 Overexpression Lysate: Product Information
This Human Tomoregulin-1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Tomoregulin-1 protein (Cat: 11005-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the human TMEFF1 (NP_003683.2) (Met1-Val330) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human TMEFF1 consists 532 amino acids and predicts a molecular mass of 58.5 kDa.
Human Tomoregulin-1 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Tomoregulin-1 HEK293 Overexpression Lysate: Alternative Names
Human C9orf2 Overexpression Lysate; Human CT120.1 Overexpression Lysate; Human H7365 Overexpression Lysate; Human TR-1 Overexpression Lysate
Tomoregulin-1 Background Information
TMEFF1 is a novel transmembrane protein, containing two follisatin domains and an epidermal growth factor-like region. The ectopic expression of TMEFF1 in brain cancer cells resulted in their growth inhibition. That TMEFF1 may behave as a tumor suppressor gene in brain cancers. TMEFF1 and TMEFF2 are putative transmembrane proteins comprised of one epidermal growth factor (EGF)-like domain and two follistatin-like domains. Both TMEFF1 and TMEFF2 are predominantly expressed in the brain.
transmembrane protein with EGF-like and two follistatin-like domains 1
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