Human TLT-1/TREML1 HEK293 Overexpression Lysate

Price:
Size:
Number:

Human TLT-1/TREML1 HEK293 Overexpression Lysate: Product Information

Product Description
This Human TLT-1/TREML1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TLT-1/TREML1 protein (Cat: 11934-H08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human TREML1 isoform 1 (Q86YW5-1) extracellular domain (Met 1-Pro 162) was expressed, with a polyhistidine tag at the C-terminus.
Molecule Mass
The recombinant human TREML1 consists of 158 amino acids and predictes a molecular mass of 17.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTREML1 is approximately 24 kDa.

Human TLT-1/TREML1 HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human TLT-1/TREML1 HEK293 Overexpression Lysate: Alternative Names

Human dJ238O23.3 Overexpression Lysate; Human GLTL1825 Overexpression Lysate; Human MGC119173 Overexpression Lysate; Human PRO3438 Overexpression Lysate; Human TLT-1 Overexpression Lysate; Human TLT1 Overexpression Lysate; Human UNQ1825/PRO3438 Overexpression Lysate

TLT-1/TREML1 Background Information

Trem-like transcript 1 protein, also known as Triggering receptor expressed on myeloid cells-like protein 1, TREML1 and TLT-1, is a cytoplasm and single-pass type I membrane protein. TREML1 / TLT-1 is expressed exclusively in platelets and megakaryocytes (MKs) and that its expression is up-regulated dramatically upon platelet activation. It is a receptor that may play a role in the innate and adaptive immune response. TREML1 / TLT-1 contains the characteristic single V-set immunoglobulin (Ig) domain, its longer cytoplasmic tail is composed of both a proline-rich region and an immune receptor tyrosine-based inhibitory motif, the latter known to be used for interactions with protein tyrosine phosphatases. The triggering receptors expressed on myeloid cells (TREMs) have drawn considerable attention due to their ability to activate multiple cell types within the innate immune system, including neutrophils, monocyte / macrophages, and dendritic cells, via their association with DAP12. TREML1 / TLT-1 is prepackaged, along with CD62P, into both MK and platelet alpha-granules. Differences in thrombin-induced redistribution of CD62P and TREML1 indicate that TREML1 is not simply cargo of alpha-granules but may instead regulate granule construction or dispersal. TREML1 / TLT-1 does not function to inhibit members of the TREM family but instead may play a role in maintaining vascular hemostasis and regulating coagulation and inflammation at sites of injury.
Full Name
triggering receptor expressed on myeloid cells like 1
References
  • Washington A.V., et al., 2002, Blood 100:3822-4.
  • Allcock R.J.N., et al., 2003, Eur. J. Immunol. 33:567-77.
  • Washington A.V., et al., 2004, Blood 104:1042-7.
  • Barrow A.D., et al., 2004, J. Immunol. 172:5838-42.
  • Gattis J.L., et al., 2006,J. Biol. Chem. 281:13396-403.
Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.