Human Transferrin Receptor/TFRC HEK293 Overexpression Lysate: Product Information
This Human Transferrin Receptor/TFRC overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Transferrin Receptor/TFRC protein (Cat: 11020-H07H) from the overexpression lysate was verified.
A DNA sequence encoding the extracellular domain of human TFRC (NP_003225.2) (Cys 89-Phe 760) was expressed with a polyhistidine tag at the N-terminus.
The recombinant human TFRC comprises 689 amino acids and has a predicted molecular mass of 77.4 kDa.
Human Transferrin Receptor/TFRC HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Transferrin Receptor/TFRC HEK293 Overexpression Lysate: Alternative Names
Human CD71 Overexpression Lysate; Human p90 Overexpression Lysate; Human T9 Overexpression Lysate; Human TFR Overexpression Lysate; Human TFR1 Overexpression Lysate; Human TR Overexpression Lysate; Human TRFR Overexpression Lysate
Transferrin Receptor/TFRC Background Information
Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein which belongs to thepeptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
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