Human TAOK3 Baculovirus-Insect cells Overexpression Lysate: Product Information
This Human TAOK3 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of TAOK3 protein (Cat: 11547-H20B) from the overexpression lysate was verified.
A DNA sequence encoding the amino acids (Met 1-Asp 411) of human TAOK3 (Q9H2K8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
The recombinant human TAOK3 (aa 1-411)/GST chimera consists of 648 amino acids and has a calculated molecular mass of 74.3 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
Human TAOK3 Baculovirus-Insect cells Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human TAOK3 Baculovirus-Insect cells Overexpression Lysate: Alternative Names
Human DPK Overexpression Lysate; Human JIK Overexpression Lysate; Human MAP3K18 Overexpression Lysate
TAOK3 Background Information
Serine/threonine-protein kinase TAO3, also known as cutaneous T-cell lymphoma-associated antigen HD-CL-9, CTCL-associated antigen HD-CL-9, Dendritic cell-derived protein kinase, JNK / SAPK-inhibitory kinase, Jun kinase-inhibitory kinase, Kinase from chicken homolog A, Thousand and one amino acid protein 3, JIK, TAOK3 and MAP3K18, is cytoplasm and peripheral membrane protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE2 subfamily. Protein kinases are pivotal regulators of cell signaling that modulate each other's functions and activities through site-specific phosphorylation events. TAOK3 / JIK contains oneprotein kinase domain. TAOK3 / JIK is ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. TAOK3 / JIK inhibits the basal activity of Jun kinase. It is negatively regulated by epidermal growth factor (EGF). When overexpressed, TAOK3 / JIK may activate ERK1 / ERK2 and JNK / SAPK.
TAO kinase 3
Tassi E., et al., 1999, J. Biol. Chem. 274:33287-95.
Yoneda T., et al., 2001, J. Biol. Chem. 276:13935-40.
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