Human TAFA2/FAM19A2 HEK293 Overexpression Lysate: Product Information
This Human TAFA2/FAM19A2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of TAFA2/FAM19A2 protein (Cat: 11169-H01H) from the overexpression lysate was verified.
A DNA sequence encoding the human FAM19A2 (Q8N3H0-1) (Ala31-His131) was expressed, fused with the Fc region of human IgG1 at the N-terminus.
The recombinant human FAM19A2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 361 amino acids and has a predicted molecular mass of 39.7 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions.
Human TAFA2/FAM19A2 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human TAFA2/FAM19A2 HEK293 Overexpression Lysate: Alternative Names
Human TAFA-2 Overexpression Lysate; Human TAFA2 Overexpression Lysate
TAFA2/FAM19A2 Background Information
FAM19A2 belongs to the FAM19/TAFA family. FAM19/TAFA family members are chemokine-like proteins. The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS. Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells. And third, TAFAs may control axonal sprouting following brain injury. Human FAM19A2 is 97% aa identical to mouse FAM19A2 and is expressed in the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus, however its expression in the CNS is 5 to 1 fold higher than in other tissues. FAM19A2 gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins.
family with sequence similarity 19 (chemokine (C-C motif)-like), member A2
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