Human ST2/IL-1 RL1 HEK293 Overexpression Lysate

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Human ST2/IL-1 RL1 HEK293 Overexpression Lysate: Product Information

Product Description
This Human ST2/IL-1 RL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of ST2/IL-1 RL1 protein (Cat: 10105-H02H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human IL1RL1 isoform 2 (NP_003847.2) (Met 1-Phe 328) was fused with the Fc region of human IgG1 at the C-terminus.
Molecule Mass
The recombinant human IL1RL1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 548 amino acids and has a calculated molecular mass of 67.1 kDa. As a result of glycosylation, the apparent molecular mass of rh IL1RL1/Fc monomer is approximately 85-95 kDa in SDS-PAGE under reducing conditions.

Human ST2/IL-1 RL1 HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human ST2/IL-1 RL1 HEK293 Overexpression Lysate: Alternative Names

Human DER4 Overexpression Lysate; Human FIT-1 Overexpression Lysate; Human IL1RL1 Overexpression Lysate; Human IL33R Overexpression Lysate; Human IL‑1 R4 Overexpression Lysate; Human ST2 Overexpression Lysate; Human ST2L Overexpression Lysate; Human ST2V Overexpression Lysate; Human T1 Overexpression Lysate

ST2/IL-1 RL1 Background Information

IL-1 receptor–like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interlukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described, and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage pro-inflammatory function. IL33 is a specific ligand of ST2L and induces production of Th2 cytokines.
Full Name
interleukin 1 receptor-like 1
References
  • Savenije OE, et al. (2011) Interleukin-1 receptor-like 1 polymorphisms are associated with serum IL1RL1-a, eosinophils, and asthma in childhood. J Allergy Clin Immunol. 127(3): 750-6.
  • Li H, et al. (2000) The cloning and nucleotide sequence of human ST2L cDNA. Genomics. 67(3): 284-90.
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