Human Src Baculovirus-Insect cells Overexpression Lysate: Product Information
This Human Src overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Src protein (Cat: 10755-H20B) from the overexpression lysate was verified.
A DNA sequence encoding the human SRC (P12931-1) (Met 1-Leu 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
The recombinant human SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 KDa. It migrates as an approximately 81 KDa band in SDS-PAGE under non-reduced conditions.
Human Src Baculovirus-Insect cells Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Src Baculovirus-Insect cells Overexpression Lysate: Alternative Names
Human ASV Overexpression Lysate; Human c-SRC Overexpression Lysate; Human p60-Src Overexpression Lysate; Human SRC1 Overexpression Lysate
Src Background Information
Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.
SRC proto-oncogene, non-receptor tyrosine kinase
Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189.
Czernilofsky AP. et al., 1980, Nature. 287: 198-203.
Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.
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