Human SR-BI/SCARB1 HEK293 Overexpression Lysate: Product Information
This Human SR-BI/SCARB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SR-BI/SCARB1 protein (Cat: 11069-H03H) from the overexpression lysate was verified.
A DNA sequence encoding the human SCARB1 (NP_005496.4) extracellular domain (Pro 33-Tyr 443) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
The recombinant human SCARB1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 659 amino acids and has a predicted molecular mass of 78 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSCARB1/Fc monomer is approximately 110-115 kDa due to glycosylation.
Human SR-BI/SCARB1 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human SR-BI/SCARB1 HEK293 Overexpression Lysate: Alternative Names
Human CD36L1 Overexpression Lysate; Human CLA-1 Overexpression Lysate; Human CLA1 Overexpression Lysate; Human HDLQTL6 Overexpression Lysate; Human SR-BI Overexpression Lysate; Human SRB1 Overexpression Lysate
SR-BI/SCARB1 Background Information
Scavenger receptor class B, member 1 (SCARB1), also known as CD36L1, is a member of the scavenger receptor family. SCARB1 is expressed primarily in liver and non placental steroidogenic tissues, and predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane. SCARB1 is proposed as a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells, and is involved in a wide variety of physilogical processes. As a key component in the reverse cholesterol transport pathway, SCARB1 binds high density lipoproteins (HDLs) and mediates selective cholesterol uptake by a mechanism distinct from the LDL pathway.High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SCARB1 may thus serve as a useful marker that predicts variation in baseline lipid levels and postprandial lipid response. The mouse SCARB1 has been shown to exert actions in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.
scavenger receptor class B, member 1
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Williams, D.L. et al., 2001, Endocr. Res. 26 (4): 639-651.
Bulte, B. S. et al., 2002, J. Biol. Chem. 277 (39): 36092-36099.
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