Human SPINK4 HEK293 Overexpression Lysate: Product Information
This Human SPINK4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SPINK4 protein (Cat: 11669-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human SPINK4 (NP_055286.1) (Met 1-Cys 86) was expressed, fused with a polyhistidine tag at the C-terminus.
The recombinant human SPINK4 consists of 71 amino acids and has a predicted molecular mass of 8 kDa. It migrates as an approximately 10 kDa band in SDS-PAGE under reducing conditions.
Human SPINK4 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human SPINK4 HEK293 Overexpression Lysate: Alternative Names
Human HEL136 Overexpression Lysate; Human MGC133107 Overexpression Lysate; Human PEC-60 Overexpression Lysate; Human PEC60 Overexpression Lysate; Human SPINK4 Overexpression Lysate
SPINK4 Background Information
Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-6 homolog and SPINK4, is a secreted protein which contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.
serine peptidase inhibitor, Kazal type 4
Schneider, A. et al., 2004,Gastroenterol Clin North Am. 33 (4): 789-806.
Wapenaar, MC. et al., 2007, Immunogenetics. 59 (5): 349-57.
Brattsand, M. et al., 2009, J Invest Dermatol. 129 (7): 1656-65.
Successfully added to cart Please enter catalog numberSubmitted successfullyNetwork ErrorPlease enter your company namePlease enter your namePlease enter your emailPlease enter a valid email addressPlease enter some messageNot found.