Human SLAMF6 HEK293 Overexpression Lysate: Product Information
This Human SLAMF6 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLAMF6 protein (Cat: 11945-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the human SLAMF6 (Q96DU3-1) (Met1-Met226) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human SLAMF6/Fc is a disulfide-linked homodimer. The reduced monomer comprises 446 amino acids and has a predicted molecular mass of 50.1 kDa. The apparent molecular mass of the protein is approximately 63-68 kDa in SDS-PAGE under reducing conditions.
Human SLAMF6 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human SLAMF6 HEK293 Overexpression Lysate: Alternative Names
Human CD352 Overexpression Lysate; Human KALI Overexpression Lysate; Human KALIb Overexpression Lysate; Human Ly108 Overexpression Lysate; Human NTB-A Overexpression Lysate; Human NTBA Overexpression Lysate; Human SF2000 Overexpression Lysate
SLAMF6 Background Information
SLAM family member 6, also known as Activating NK receptor, NK-T-B-antigen, NTB-A, SLAMF6, KALI and Ly18, is a single-pass type I membrane protein which belongs to the CD2 subfamily of the immunoglobulin superfamily. SLAMF6 / Ly18 contains one Ig-like (immunoglobulin-like) domain. It is expressed by all (resting and activated) natural killer cells (NK), T- and B-lymphocytes. SLAMF6 / Ly18 triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. SLAMF6 / Ly18 is a homodimer. It interacts with PTN6 and, upon phosphorylation, with PTN11 and SH2D1A/SAP. SLAMF6 / Ly18 undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It may function as a coreceptor in the process of NK cell activation. SLAMF6 / Ly18 can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients.
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