Human Relaxin 1/RLN1 HEK293 Overexpression Lysate: Product Information
This Human Relaxin 1/RLN1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Relaxin 1/RLN1 protein (Cat: 11625-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human RLN1 (NP_008842.1) (Met 1-Cys 185) was fused with a polyhistidine tag at the C-terminus.
The secreted pro form of recombinant human RLN1 consists of 174 amino acids and predictes a molecular mass of 20 kDa as estimated in SDS-PAGE under reducing conditions.
Human Relaxin 1/RLN1 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Relaxin 1/RLN1 HEK293 Overexpression Lysate: Alternative Names
Human bA12D24.3.1 Overexpression Lysate; Human bA12D24.3.2 Overexpression Lysate; Human H1 Overexpression Lysate; Human H1RLX Overexpression Lysate; Human RLN1 Overexpression Lysate; Human RLXH1 Overexpression Lysate
Relaxin 1/RLN1 Background Information
Relaxin-1, also known as Prorelaxin H1 and RLN1, is a secreted protein which belongs to theinsulin family. It is a peptide hormone that was first described in 1926 by Frederick Hisaw. Since its discovery as a reproductive hormone 8 years ago, relaxin has been implicated in a number of pregnancy-related functions involving extracellular matrix (ECM) turnover and collagen degradation. It is now becoming evident that relaxin's ability to reduce matrix synthesis and increase ECM degradation has important implications in several nonreproductive organs, including the heart, lung, kidney, liver and skin. The relaxin-like peptide family belongs in the insulin superfamily and consists of 7 peptides of high structural but low sequence similarity; relaxin-1 (RNL1), relaxin-2 (RNL2) and relaxin-3 ( RNL3), and the insulin-like (INSL) peptides, INSL3, INSL4, INSL5 and INSL6. The functions of relaxin-3, INSL4, INSL5, INSL6 remain uncharacterised. Relaxin-1 / RLN1 is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. Relaxin-1 / RLN1 may be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Relaxin and estrogen appear to play protective roles against airway fibrosis, airway SM thickening, and cardiac hypertrophy. Relaxin may also provide a means to regulate excessive collagen deposition during kidney development and in diseased states characterized by renal fibrosis.
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Samuel,C.S. et al., 2003, Curr Opin Pharmacol. 3 (2):152-8.
Samuel,C.S. et al., 2004,Kidney Int. 65 (6):2054-64.
Lekgabe,E.D. et al., 2006, Endocrinology. 147 (12):5575-83.
Samuel,C.S. et al., 2007, Adv Exp Med Biol 612: 88-103.
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