Human PCTAIRE1 Baculovirus-Insect cells Overexpression Lysate: Product Information
This Human PCTAIRE1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of PCTAIRE1 protein (Cat: 10631-H09B) from the overexpression lysate was verified.
A DNA sequence encoding the human PCTK1 (Q00536-1) (Met 1-Phe 496) was fused with the GST tag at the N-terminus.
The recombinant human PCTK1/GST chimera consists of 720 amino acids and has a predicted molecular mass of 82 kDa. It migrates as an approximately 73 kDa band in SDS-PAGE under reducing conditions.
Human PCTAIRE1 Baculovirus-Insect cells Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
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