Human MKK6 Baculovirus-Insect cells Overexpression Lysate: Product Information
This Human MKK6 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of MKK6 protein (Cat: 10422-HNCB1) from the overexpression lysate was verified.
A DNA sequence encoding the human MAP2K6 (P52564-1) (Met 1-Asp 334, 207Asp, 211Asp) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
The secreted recombinant human MAP2K6 consists of 336 amino acids and predicts a molecular mass of 37.7 KDa. The apparent molecular mass of the protein is approximately 38 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Human MKK6 Baculovirus-Insect cells Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human MKK6 Baculovirus-Insect cells Overexpression Lysate: Alternative Names
Human MAPKK6 Overexpression Lysate; Human MEK6 Overexpression Lysate; Human MKK6 Overexpression Lysate; Human PRKMK6 Overexpression Lysate; Human SAPKK-3 Overexpression Lysate; Human SAPKK3 Overexpression Lysate
MKK6 Background Information
Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the?protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
mitogen-activated protein kinase kinase 6
Raingeaud J., et al.,(1996), MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Mol. Cell. Biol. 16:1247-1255.
Stein B., et al., (1996), Cloning and characterization of MEK6, a novel member of the mitogen-activated protein kinase kinase cascade.J. Biol. Chem. 271:11427-11433.
Han J., et al.,(1996), Characterization of the structure and function of a novel MAP kinase kinase (MKK6).J. Biol. Chem. 271:2886-2891.
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