Human LAIR2 HEK293 Overexpression Lysate: Product Information
This Human LAIR2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LAIR2 protein (Cat: 10868-HNAH-E) from the overexpression lysate was verified.
A DNA sequence encoding the human LAIR2 (NP_002279.2) (Met 1-Pro 152) was expressed and purified.
The secreted recombinant human LAIR2 comprises 131 amino acids with a predicted molecular mass of 14.1 kDa. As a result of glycosylation, the apparent molecular mass of rh LAIR2 is approximately 20 kDa in SDS-PAGE under reducing conditions.
Human LAIR2 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human LAIR2 HEK293 Overexpression Lysate: Alternative Names
Human CD306 Overexpression Lysate; Human LAIR2 Overexpression Lysate; Human MGC71634 Overexpression Lysate; Human XXbac-BCX535A19.6 Overexpression Lysate
LAIR2 Background Information
Leukocyte-associated immunoglobulin-like receptor 2 ( LAIR2 ), also known as CD36, is a 131 amino acid protein containing one lg-like C2-type domain. It is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 is thought to be secreted and may help modulate mucosal tolerance. As a natural competitor for LAIR1, soluble LAIR2 prevents binding of human LAIR1 to collagens and LAIR1 cross-linking, thereby regulating its inhibitory potential. Accordingly, LAIR2 is suggested to perform an immunoregulatory function.
leukocyte associated immunoglobulin like receptor 2
Meyaard, L. et al.,1997, Immunity.7:283-290.
Meyaard, L. et al.,1999, J. Immunol. 162:5800-5804.
Meyaard, L. et al., 2001, J. Exp. Med. 194 (1): 107-112.
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