Human Interferon alpha 10/IFNA10 HEK293 Overexpression Lysate: Product Information
This Human Interferon alpha 10/IFNA10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Interferon alpha 10/IFNA10 protein (Cat: 10349-H01H) from the overexpression lysate was verified.
A DNA sequence encoding the human IFNA10 (P01566) (Cys 24-Asp 189) was fused with the Fc region of human IgG1 at the N-terminus.
The recombinant human IFNA10/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 427 amino acids with a predicted molecular mass of 48 kDa. AsrhIFNA10/Fc monomer migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Human Interferon alpha 10/IFNA10 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Interferon alpha 10/IFNA10 HEK293 Overexpression Lysate: Alternative Names
Human IFN-alphaC Overexpression Lysate; Human MGC119878 Overexpression Lysate; Human MGC119879 Overexpression Lysate
Interferon alpha 10/IFNA10 Background Information
Interferon alpha-1 (IFNA1) is a member of the interferon family. Interferons belong to the group of the regulatory glycoproteins, of low molecular mass. They are the products of infected cell-genome, but not virus, as a consequence of the cause answer by different inductors. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Human IFN are divided on the sequence of amino-acids into three groups: Alpha, Beta and Gamma interferons.
interferon, alpha 10
De Veer MJ, et al. (2001) Functional classification of interferon-stimulated genes identified using microarrays. J Leukoc Biol. 69 (6): 912-20.
Liu YJ. (2005) IPC: professional type 1 interferon-producing cells and plasmacytoid dendritic cell precursors. Annu Rev Immunol. 23: 275-306.
Fensterl V, et al. (2009) Interferons and viral infections. Biofactors. 35 (1): 14-20.
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