Human Integrin beta 1 HEK293 Overexpression Lysate

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Human Integrin beta 1 HEK293 Overexpression Lysate: Product Information

Product Description
This Human Integrin beta 1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Integrin beta 1 protein (Cat: 10587-H08H1) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human ITGB1 (CAA30790.1) (Met1-Asp728) was expressed with a polyhistidine tag at the C-terminus.
Molecule Mass
The recombinant human ITGB1 consists 749 amino acids and predicts a molecular mass of 83.3 kDa.

Human Integrin beta 1 HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human Integrin beta 1 HEK293 Overexpression Lysate: Alternative Names

Human CD29 Overexpression Lysate; Human FNRB Overexpression Lysate; Human GPIIA Overexpression Lysate; Human MDF2 Overexpression Lysate; Human MSK12 Overexpression Lysate; Human VLA-BETA Overexpression Lysate; Human VLAB Overexpression Lysate

Integrin beta 1 Background Information

ITGB1 is a heterodimeric cell-surface receptor involved in cell functions such as proliferation, migration, invasion and survival.Integrin beta1 (ITGB1) has been recognized to play a major role in tumor growth, invasion and metastasis.Using luciferase assays, we identified integrin beta1 (ITGB1) as a direct target of miR-134.ITGB1 is a direct target of miR-493-5p suggesting that ITGB1 and miR-493-5p may have potential prognostic value and may be useful as tumor biomarkers for the diagnosis of NSCLC patients.
Full Name
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
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