Human IL20RA HEK293 Overexpression Lysate

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Human IL20RA HEK293 Overexpression Lysate: Product Information

Product Description
This Human IL20RA overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL20RA protein (Cat: 10397-H08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the extracellular domain of human IL20Rα isoform 1 (NP_055247.3) (Met 1-Lys 250) was fused with a polyhistidine tag at the C-terminus.
Molecule Mass
The recombinant human IL20Rα consists of 232 amino acids and has a calculated molecular mass of 26.8 kDa. As a result of glycosylation, rhIL20Rα migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.

Human IL20RA HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human IL20RA HEK293 Overexpression Lysate: Alternative Names

Human CRF2-8 Overexpression Lysate; Human IL-20R-alpha Overexpression Lysate; Human IL-20R1 Overexpression Lysate; Human IL-20RA Overexpression Lysate

IL20RA Background Information

Interleukin 2 receptor, alpha subunit (IL2RA/IL-2RA) also known as IL-2 receptor subunit alpha, Cytokine receptor class-II member 8, interleukin-2 receptor I, and interleukin-2 receptor subunit alpha, is a subunit for the interleukin-2 receptor. IL2RA/IL-2RA belongs to the type II cytokine receptor family. This cytokine is a receptor for interleukin 2 (IL2), a cytokine that may be involved in epidermal function. The receptor of IL2 is a heterodimeric receptor complex consisting of this protein and interleukin 2 receptor beta (IL2B). IL2RA forms heterodimer with IL2RB, and the complex serves as a receptor for IL19, IL2 and IL24. All three are capable of signaling through IL-2RA/IL-2RB complex. The ligand binding to receptor B creating a high-affinity binding site for the receptor A which is recruited to complete the complex. In addition, IL2RA also forms a heterodimer with the unique and specific receptor IL1RB and functions as the receptor for IL26. IL2RA is widely expressed with highest levels in skin, testis and brain. The expression of both IL2RA and IL2RB is found to be upregulated in psoriatic skin lesions on keratinocytes.
Full Name
interleukin 20 receptor, alpha
References
  • Parrish-Novak J, et al. (2002) Interleukins 19, 20, and 24 signal through two distinct receptor complexes. Differences in receptor-ligand interactions mediate unique biological functions. J Biol Chem. 277(49): 47517-23.
  • Kingo K, et al. (2008) Association analysis of IL20RA and IL20RB genes in psoriasis. Genes Immun. 9(5): 445-51.
  • Blumberg H, et al. (2001) Interleukin 20: discovery, receptor identification, and role in epidermal function. Cell. 104(1): 9-19.
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