Human IL17RC HEK293 Overexpression Lysate

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Human IL17RC HEK293 Overexpression Lysate: Product Information

Product Description
This Human IL17RC overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL17RC protein (Cat: 11747-H02H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the extracellular domain of human IL17RC isoform 3 (NP_116121.2) (Met 1-Ala 454) was fused with the Fc region of human IgG1 at the C-terminus.
Molecule Mass
The secreted recombinant human IL17RC/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 675 amino acids and has a predicted molecular mass of 75.3 kDa. The apparent molecular mass of rh IL17RC/Fc monomer is approximately 100-120 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Human IL17RC HEK293 Overexpression Lysate: Usage Guide

Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human IL17RC HEK293 Overexpression Lysate: Alternative Names

Human IL17-RL Overexpression Lysate; Human IL17RL Overexpression Lysate; Human UNQ6118/PRO20040/PRO38901 Overexpression Lysate

IL17RC Background Information

The hypomethylation within the IL17RC gene promoter in peripheral blood is not suitable for use as a clinical biomarker of AMD. This study highlights the need for considerable replication of epigenetic association studies prior to clinical application. methylation of IL17RC could play as a marker in choroidal neovascularization (CNV) and degeneration of retinal pigment epithelium (RPE) cells in vitro.
Full Name
interleukin 17 receptor C
References
  • Allen W, et al. (2010) IL-17RC: A partner in IL-17 signaling and beyond. Semin Immunopathol. 32(1): 33-42.
  • You ZB, et al. (2007) Differential Expression of IL-17RC Isoforms in Androgen-Dependent and Androgen-Independent Prostate Cancers1. Neoplasia. 9(6): 464-70.
  • Zrioual S, et al. (2008) IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood. J Immunol. 180(1): 655-63.
  • Kuestner RE, et al. (2010) Identification of the IL-17 Receptor Related Molecule IL-17RC as the Receptor for IL-17F. J Immunol.179(8): 5462-73.
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